High expression of muscarinic acetylcholine receptor 3 predicts poor prognosis in patients with pancreatic ductal adenocarcinoma.
High expression of muscarinic acetylcholine receptor 3 predicts poor prognosis in patients with pancreatic ductal adenocarcinoma.
复制标题
毒蕈碱乙酰胆碱受体3的高表达预示胰腺导管腺癌患者预后不良
DOI：
10.2147/ott.s111382
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发表时间：
2016
影响因子：
4
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Zhang L;Xiu D;Zhan J;He X;Guo L;Wang J;Tao M;Fu W;Zhang H
目的近年来的研究表明，M受体3(M3)作为M受体家族成员之一，在正常生理功能中发挥着重要作用，参与肿瘤的发生发展。然而，M3在胰腺导管腺癌中的作用尚不清楚。本研究的目的是探讨M3在PDAC中的表达及其预后价值。材料与方法用免疫组织化学方法检测M3在PDAC中的定位和表达。用VACHT法检测相应M3PDAC组织中的副交感神经纤维。采用Kaplan-Meier分析评估M3表达与患者生存的相关性。结果M3主要定位于细胞质，在所有PDAC患者标本中均有表达。M3信号的增强与PDAC的高级别(P<；0.01)、更多的淋巴结转移(P<；0.01)以及患者总的生存时间(P<；0.01)显著相关。形态上，高表达M3的细胞多位于浸润性肿瘤前沿/肿瘤萌芽细胞、转移性淋巴结和副交感神经纤维。结论M3高表达可作为预测PDAC预后的指标。
Aims Recent studies showed that muscarinic acetylcholine receptor 3 (M3), as a muscarinic acetylcholine receptor family member that plays an important role in normal physiological function, is engaged in cancer progression. However, the role of M3 in pancreatic ductal adenocarcinoma (PDAC) is not known. The aim of this study is to investigate the expression and prognostic value of M3 in patients with PDAC. Materials and methods The localization and expression of M3 in PDAC were examined by immunohistochemistry. VAChT was employed to detect parasympathetic nerve fibers in the corresponding M3 PDAC tissues. The correlation between M3 expression and patients’ survival was assessed by Kaplan–Meier analysis. Results M3 was discovered predominantly localized in the cell cytoplasm and expressed in all specimens of PDAC patients. Significant correlation was noted between increased M3 intensity and high grade of PDAC (P<0.01), more lymph node metastasis (P<0.01) as well as shorter patient overall survival (P<0.01). Morphologically, cells with high M3 expression were more frequently located at the invasive tumor front/tumor budding cells, metastatic lymph nodes and parasympathetic nerve fibers. Conclusion High expression of M3 is a prognostic marker for PDAC.