Expression of COX-2, PCNA, Ki-67 and p53 in gastrointestinal stromal tumors and its relationship with histopathological parameters
Expression of COX-2, PCNA, Ki-67 and p53 in gastrointestinal stromal tumors and its relationship with histopathological parameters
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DOI：
10.3748/wjg.v13.i3.426
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发表时间：
2007-01-21
影响因子：
4.3
通讯作者：
Tuncer, Ilhan
中科院分区：
文献类型：
--
作者：
Gumurdulu, Derya;Erdogan, Seyda;Tuncer, Ilhan
目的：探讨环氧合酶-2(COX-2)、增殖细胞核抗原(PCNA)、Ki-67和P53在胃肠道间质瘤中的表达及其与组织病理学参数的关系。采用免疫组织化学方法检测C-KIT、CD34、SMA、S-100蛋白、COX-2、增殖细胞核抗原、Ki-67和P53蛋白的表达，并分析其与核分裂指数(MI)、肿瘤分级、肿瘤大小、COX-2、增殖细胞核抗原、Ki-67和p53蛋白表达的关系。结果：COX-2蛋白阳性表达19例(76%)，阳性表达位于肿瘤细胞浆中。结论：COX-2在大多数GIST中均有表达，COX-2可能在GIST的增殖和发展中起重要作用，也可能是鉴别GIST的有用指标，但P53的免疫组织化学检测可用于区分GIST的危险组，肿瘤大小和有丝分裂率应同时考虑。(C)2007年WJG出版社。版权所有。
AIM: To investigate the expression of Cyclooxygenase-2 (COX-2), proliferating cell nuclear antigen (PCNA), Ki-67 and p53 in gastrointestinal stromal tumors (GISTs) and its relationship with histopathological parameters.METHODS: Twenty-five GISTs were examined by light microscopy and immunohistochemistry. c-kit, CD34, SMA, S-100 protein, COX-2, PCNA, Ki-67 and p53 were detected immunohistochemically and the relationship was evaluated among histopathologic parameters such as mitotic index (MI), tumor grade, tumor size, COX-2, PCNA, Ki-67 and p53.RESULTS: COX-2 protein expression was found in 19 of 25 (76%) of the tumors, and expression was noted in the cytoplasm of the tumor cells. p53 was significantly related to MI and tumor grade but no relationship was found between COX-2, proliferation markers and MI, tumor grade and tumor size.CONCLUSION: COX-2 is expressed in most GISTs and it may play an important role in the proliferation and progression of these tumors or a useful marker to identify GIST Although immunohistochemical assessment of p53 can be used for distinguishing the risk groups of GISTs, tumor size and mitotic rate should be considered at the same time. (c) 2007 The WJG Press. All rights reserved.