Identification of major loci and genomic regions controlling acid and volatile content in tomato fruit: implications for flavor improvement
Identification of major loci and genomic regions controlling acid and volatile content in tomato fruit: implications for flavor improvement
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DOI：
10.1111/nph.14615
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发表时间：
2017-07-01
期刊：
影响因子：
9.4
通讯作者：
Causse, Mathilde
中科院分区：
文献类型：
--
作者：
Bauchet, Guillaume;Grenier, Stephane;Causse, Mathilde
植物代谢物对作物产量和营养品质的影响对世界粮食安全至关重要，本文报道了300个番茄品种（Solanum lycopersicum和相关野生种）在2年内的代谢特征，通过定量测定60种初级和次级代谢物，包括挥发性有机化合物。利用10000个单核苷酸多态性标记的基因型信息，进行了代谢物全基因组关联作图（genome-wide association mapping，GWAS）研究。我们确定了79种影响13种初级代谢物和19种次级代谢物的关联，在高分辨率下具有较大的影响。检测到四个基因组区域，突出显示了控制几种代谢物变异的关联簇。局部连锁不平衡分析和等位基因挖掘确定了可能的候选基因，这可能会调节代谢产物的含量，是人类的饮食和水果consumption.We显着的重要性，精确地描述了两个协会参与水果酸度和苯丙烷类挥发物的生产。总之，这项研究揭示了番茄亚种中复杂而独特的代谢物调控，并表明GWAS是基因代谢物注释和鉴定、途径阐明和进一步作物改良的有力工具。
Plant metabolites are important to world food security due to their roles in crop yield and nutritional quality.Here we report the metabolic profile of 300 tomato accessions (Solanum lycopersicum and related wild species) by quantifying 60 primary and secondary metabolites, including volatile organic compounds, over a period of 2 yr. Metabolite content and genetic inheritance of metabolites varied broadly, both within and between different genetic groups.Using genotype information gained from 10 000 single nucleotide polymorphism markers, we performed a metabolite genome-wide association mapping (GWAS) study. We identified 79 associations influencing 13 primary and 19 secondary metabolites with large effects at high resolution. Four genome regions were detected, highlighting clusters of associations controlling the variation of several metabolites. Local linkage disequilibrium analysis and allele mining identified possible candidate genes which may modulate the content of metabolites that are of significant importance for human diet and fruit consumption.We precisely characterized two associations involved in fruit acidity and phenylpropanoid volatile production. Taken together, this study reveals complex and distinct metabolite regulation in tomato subspecies and demonstrates that GWAS is a powerful tool for gene-metabolite annotation and identification, pathways elucidation, and further crop improvement.