High-resolution genetical and physical mapping of the Rx gene for extreme resistance to potato virus X in tetraploid potato
High-resolution genetical and physical mapping of the Rx gene for extreme resistance to potato virus X in tetraploid potato
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DOI：
10.1007/s001220050543
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发表时间：
1997-07-01
影响因子：
5.4
通讯作者：
Baulcombe, DC
中科院分区：
文献类型：
--
作者：
Bendahmane, A;Kanyuka, K;Baulcombe, DC
马铃薯中的Rx位点对PVX具有极强的抗性。在PVX敏感品种Huinkel与品种Cara （Rx基因型）杂交的F-1后代中，出现了1:1的PVX抗性分离，说明Cara的Rx在单一条件下存在。利用马铃薯和番茄的RFLP标记，我们将Cara的Rx定位到与先前定位的Rx1位点不同的第XII染色体远端。为了在Rx附近生成高分辨率的连锁图谱，使用大量抗性和易感分离的DNA筛选了总共728个AFLP引物组合。我们还筛选了与Rx位点相关的染色体重组事件的分离群体，并鉴定出82株与Rx位点接近的重组事件。利用这些重组植物，我们已经确定了位于Rx侧面的aflp，在基因组的一个区域中，1 cM相当于大约400kb，其间隔为0.23 cM。
The Rx locus in potato confers extreme resistance to PVX. In the F-1 progeny of crosses between the PVX-susceptible cultivar Huinkel and the cultivar Cara (Rx genotype) there was a 1:1 segregation of PVX resistance, indicating that Rx in Cara is present in the simplex condition. Using potato and tomato RFLP markers, we mapped Rx in Cara to the distal end of chromosome XII at a different position to the previously mapped Rx1 locus. To generate a high-resolution linkage map in the vicinity of Rx a total 728 AFLP primer combinations were screened using DNA of bulked resistant and susceptible segregants. We also screened segregating populations for chromosomal recombination events linked to the Rx locus and identified 82 plants with recombination events close to Rx. Using these recombinant plants we have identified AFLPs that flank Rx and span an interval of 0.23 cM in a region of the genome where 1 cM corresponds to approximately 400 kb.