Design and in vitro evaluation of simvastatin-hydroxyapatite coatings by an electrochemical process on titanium surfaces.
Design and in vitro evaluation of simvastatin-hydroxyapatite coatings by an electrochemical process on titanium surfaces.
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DOI：
10.1166/jbn.2014.1859
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发表时间：
2014-07
影响因子：
2.9
通讯作者：
Z. Shifang;S. Jue;H. Fuming;Li Liu;Y. Guoli
中科院分区：
文献类型：
--
作者：
Z. Shifang;S. Jue;H. Fuming;Li Liu;Y. Guoli
本研究的目的是通过电化学方法在钛表面存款辛伐他汀-羟基磷灰石（sim-HA）涂层，并评估骨祖细胞对sim-HA涂层表面的反应。将辛伐他汀以不同浓度（10（-7）、10（-6）、10（-5）和10（-4）mol/L）涂于钛表面。通过FSEM、XRD和FTIR对样品进行了表征。采用LC-MS/MS法定量Sim-HA涂层表面中的辛伐他汀。以小鼠颅骨来源的前成骨细胞（MC 3 T3-E1）的增殖、碱性磷酸酶活性（ALP）和骨钙素释放来测定成骨活性。FSEM观察表明，棒状HA晶体覆盖在所有表面上。随着药物浓度的增加，晶体直径减小。XRD和FTIR观察表明所有涂层均由HA组成。LC-MS/MS检测显示10（-6）mol/L组辛伐他汀浓度为2.77 × 10（-7）mol/L/cm 2，而10（-7）mol/L组为1.89 × 10（-7）mol/L/cm 2。MC 3 T3-E1细胞在10（-7）mol/L和10（-6）mol/L Sim-HA表面上生长，第7天ALP活性较HA组明显升高（P < 0.05），第10天各Sim-HA组ALP活性均较HA组明显升高（P < 0.05）。两个Sim-HA组在第7、10和14天骨钙素含量均显着增加（P < 0.05）。结果显示，10（-7）mol/L辛伐他汀孵育7 d后，与HA组相比，细胞增殖明显增强（P < 0.05）。结果表明，辛伐他汀可通过电化学方法成功地沉积到HA涂层中，sim-HA涂层可促进骨祖细胞的分化。
The aim of this study was to deposit simvastatin-hydroxyapatite (sim-HA) coatings on titanium surfaces by an electrochemical process and evaluate osteoprogenitor cell responses to sim-HA-coated surfaces. Simvastatin was prepared onto titanium surfaces with varying concentration (10(-7), 10(-6), 10(-5) and 10(-4) mol/L). Surface characteristics were performed by FSEM, XRD and FTIR. LC-MS/MS method was used for simvastatin quantification in Sim-HA-coated surfaces. Murine calvaria-derived pre-osteoblastic cell (MC3T3-E1) proliferation, alkaline phosphatase activity (ALP) and osteocalcin release were used to measure osteoblastic activities. FSEM observation showed rod-like HA crystals covered on all surfaces. As drug concentration increased, the crystal diameter decreased. XRD and FTIR observations showed all coatings consisted of HA. LC-MS/MS test showed the simvastatin concentration in 10(-6) mol/L group was 2.77 x 10(-7) mol/L/cm2, while in 10(-7) mol/L group was 1.89 x 10(-7) mol/L/cm2. MC3T3-E1 cells grown on 10(-7) mol/L and 10(-6) mol/L Sim-HA surfaces showed increased ALP activity as compared to HA-coated surfaces on day 7 (P < 0.05) while at 10 day, all Sim-HA groups were significantly increased than HA group (P < 0.05). Two Sim-HA groups showed significant increases in osteocalcin production on 7 10 and 14 day (P < 0.05). It showed that incubation with 10(-7) mol/L simvastatin enhanced the cell proliferation to a statistically significant extent (P < 0.05) compared with the HA group at day 7. It was concluded that simvastatin was successfully deposited into HA coatings using the electrochemical process and the sim-HA coatings enhanced differentiation of osteoprogenitor cells.