Production of wound-tumor virus and wound-tumor soluble antigen in the insect vector.
Production of wound-tumor virus and wound-tumor soluble antigen in the insect vector.
复制标题
在昆虫载体中生产伤口肿瘤病毒和伤口肿瘤可溶性抗原。
DOI：
10.1016/0042-6822(66)90130-9
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发表时间：
1966
期刊：
影响因子：
3.7
通讯作者：
L. Black
中科院分区：
文献类型：
--
作者：
D. Reddy;L. Black
通过使用实验得出的回归曲线来测量甜三叶草肿瘤（Melilotus officinalis(L.) Lam.）和昆虫载体（Agallia constricta(Van Duzee)）提取物中感染性伤口肿瘤病毒（WTV）的相对浓度，该回归曲线将注射到载体中的病毒浓度与21天后在载体中达到的伤口肿瘤可溶性抗原（WTSA）滴度相关联。 WTSA滴度通过沉淀素环时间测试测定，并且与标准WTSA制剂的滴度相关。将两种不同浓度的WTV注射到A组后。在注射较高浓度 WTV 的昆虫中，Constricta WTSA 较早达到平台水平。随后，注射较低浓度的昆虫也达到了相同的 WTSA 平台。经过 1 天的获取访问期后，A 中的 WTSA 和 WTV 浓度。从第6天到第27天急剧收缩，然后略有下降。获得后第7天到第30天，病毒浓度增加了1000倍，第30天到第45天，病毒浓度下降至峰值浓度的约十分之一。 WTV 和 WTSA 浓度的下降并不是由于假设带毒昆虫的死亡率高于无毒昆虫造成的。
Relative concentrations of inftctive wound-tumor virus (WTV) in extracts from sweet clover tumors (Melilotus officinalis(L.) Lam.) and insect vectors (Agallia constricta(Van Duzee)) were measured by using an experimentally derived regression curve relating the concentration of virus injected into vectors to the wound-tumor soluble antigen (WTSA) titer reached in them 21 days later. The WTSA titers were determined by a precipitin ring-time test and were relative to the titer of a standard WTSA preparation.After injection of two different concentrations of WTV into groups ofA. constrictathe WTSA attained a plateau level earlier in the insects injected with the higher concentrations of WTV. Later the same WTSA plateau was attained in insects injected with the lower concentrations.After a 1-day acquisition access period, the WTSA and WTV concentrations inA. constrictarose steeply from day 6 to day 27 and then decreased slightly. The virus concentration increased 1000-fold between day 7 and day 30 after acquisition, and between day 30 and day 45 it decreased to about one-tenth of the peak concentration. The drop in the WTV and WTSA concentration was not caused by a hypothetical higher mortality among viruliferous than nonviruliferous insects.