A Role of the cspA Gene Encoding a Mycolyltransferase in the Growth under Alkaline Conditions of Corynebacterium glutamicum
A Role of the cspA Gene Encoding a Mycolyltransferase in the Growth under Alkaline Conditions of Corynebacterium glutamicum
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编码霉基转移酶的cspA基因在谷氨酸棒杆菌碱性条件下生长中的作用
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发表时间：
2010
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影响因子：
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通讯作者：
M. Wachi
中科院分区：
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作者：
Ryo Takeshita;Hisao Ito;M. Wachi
谷氨酸棒杆菌广泛应用于氨基酸的工业生产。生产菌株是通过经典的随机诱变产生的，因此具有由不必要的突变引起的有害特性。增加的碱敏感性是那些不期望的特性之一。我们发现实验室菌株之一AJ12036ΔcspAΔcspB在碱性条件下显示出生长下降。为了阐明哪种突变导致碱敏感性，我们从野生型菌株ATCC 13869构建了携带ΔcspA和/或ΔcspB突变的突变菌株。我们发现，单独破坏编码分枝菌酰转移酶的cspA引起碱敏感性增加。ΔcspA突变体也显示出对乙胺丁醇、青霉素和利福平的敏感性增加。cspB的破坏对碱敏感性或药物敏感性没有影响。这些结果表明，霉菌层是重要的碱敏感性，以及药物敏感性，在这种细菌。
Corynebacterium glutamicum is widely used in the industrial production of amino acids. Producer strains are generated by classical random mutagenesis, and therefore have detrimental characteristics caused by unnecessary mutations. Increased alkali sensitivity is one of those undesired characteristics. We found that one of the laboratory strains, AJ12036ΔcspAΔcspB, showed decreased growth under alkaline conditions. To clarify which mutation is responsible for alkali sensitivity, we constructed mutant strains carrying the ΔcspA and/or ΔcspB mutations from wild-type strain ATCC13869. We found that disruption of cspA encoding a mycolyltransferase alone caused increased alkali sensitivity. The ΔcspA mutant also showed increased susceptibility to ethambutol, penicillin, and rifampicin. Disruption of cspB had no effect on alkali sensitivity or drug sensitivity. These results indicate that the mycolate layer is important for alkali sensitivity as well as drug susceptibility in this bacterium.