Incorporation of 11,12-dihydroretinal into the retinae of vitamin a deprived rats.
Incorporation of 11,12-dihydroretinal into the retinae of vitamin a deprived rats.
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将 11,12-二氢视网膜掺入维生素 A 剥夺大鼠的视网膜。
DOI：
10.1111/j.1751-1097.1981.tb04302.x
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发表时间：
1981
影响因子：
3.3
通讯作者：
Balogh-Nair,V
中科院分区：
文献类型：
--
作者：
Crouch,R;Katz,S;Nakanishi,K;Gawinowicz,MA;Balogh-Nair,V
11，12-[15- 3 H]-二氢视网膜（一种视网膜，其中关键的11-烯饱和）通过腹腔注射相应的乙醇进入维生素A缺乏大鼠的视网膜，这通过在视杆外节中存在氚标记和使用高压液相色谱法鉴定提取的视网膜来证明。由于缺乏维生素A而降低的视网膜电图（ERG）B-波振幅不受类似物给药的影响，尽管对缺乏维生素A的同窝仔进行类似的经视网膜给药后，ERG B-波振幅恢复到正常水平。（1）当与11-顺式视黄醛一起孵育时，来自维生素A缺乏大鼠的提取物再生1.4 nmol视紫红质，而来自维生素A缺乏并补充有11，12-二氢视黄醛的大鼠的提取物再生0.6 nmol视紫红质，表明二氢视黄醛的结合阻断视紫红质再生。(2)在用二氯甲烷提取后，11，12-二氢视黄醛在己烷洗涤的视网膜中显示保持不变，并且（3）将视黄醛注射到先前注射了11，12-二氢视黄醛的动物中也未能恢复通过ERG B-波测量的视觉灵敏度。我们的研究结果表明，二氢生色团占据与天然11-顺式视黄酸相同的结合位点，并且占据视蛋白的生色团结合位点不足以恢复维生素A缺乏动物的视觉灵敏度。
The incorporation of 11,12‐[15–3H]‐dihydroretinal, a retinal in which the crucial 11‐ene is saturated, into the retinae of vitamin A deficient rats as a result of intraperitoneal injection of the corresponding alcohol was shown by the presence of the tritium label in the rod outer segments and by identification of the extracted retinals using high pressure liquid chromatography. The amplitude of the electroretinogram (ERG) b‐wave, diminished as the result of vitamin A deprivation, was not affected by administration of the analogue, although similar treatment of deprived litter mates withtransretinal restored the ERG b‐wave amplitude to a normal level.The evidence that the analogue is bound to opsin forming 11,12‐dihydrorhodopsin is as follows: (1) when incubated with 11‐cisretinal, extracts from vitamin A deficient rats regenerate 1.4 nmol rhodopsin while extracts from rats deficient in vitamin A and supplemented with 11,12‐dihydroretinal regenerate 0.6 nmol rhodopsin indicating binding of the dihydroretinal blocks rhodopsin regeneration. (2) 11,12‐dihydroretinal is shown to remain unchanged in hexane‐washed retinae after extraction with methylene chloride and (3) injection of retinal into animals previously injected with 11,12‐dihydroretinal also fails to restore visual sensitivity as measured by the ERG b‐wave. Our results indicate that the dihydro‐chromophore occupies the same binding site as the natural 11‐cisretinal and that occupation of the chromophore binding site of opsin is not sufficient to restore the visual sensitivity in a vitamin‐A‐deprived animal.