Rapid Screening of Multiclass Syrup Adulterants in Honey by Ultrahigh-Performance Liquid Chromatography/Quadrupole Time of Flight Mass Spectrometry
Rapid Screening of Multiclass Syrup Adulterants in Honey by Ultrahigh-Performance Liquid Chromatography/Quadrupole Time of Flight Mass Spectrometry
复制标题
通过超高效液相色谱/四极杆飞行时间质谱快速筛查蜂蜜中的多类糖浆掺假物
DOI：
10.1021/acs.jafc.5b01410
复制
发表时间：
2015-07-29
影响因子：
6.1
通讯作者：
Cao, Wei
中科院分区：
文献类型：
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作者：
Du, Bing;Wu, Liming;Cao, Wei
蜂蜜中掺入糖浆是一个普遍存在的问题。几种类型的糖浆已被用于蜂蜜掺假，并且没有一种可用的方法能够同时检测所有这些掺杂物。在这项研究中，我们建立了一个小规模的数据库，其中包含糖浆标志物的特定色谱和质谱信息，并开发了一种简单、快速且有效的超高效液相色谱/四极杆飞行时间质谱（UHPLC/Q - TOF - MS）方法用于检测掺假蜂蜜。玉米糖浆、高果糖玉米糖浆、转化糖浆和大米糖浆被用作蜂蜜掺杂物；多糖、双果糖酐和2 - 乙酰呋喃 - 3 - 吡喃葡萄糖苷被用作检测标志物。蜂蜜中10%的糖浆含量可以很容易地被检测到。
Honey adulteration with sugar syrups is a widespread problem. Several types of syrups have been used in honey adulteration, and there is no available method that on simultaneously detect all of these adulterants. In this study, we generated a small-scale database containing the specific chromatographic and:mass spectrometry information on sugar syrup markers and developed a simple, rapid, and effective ultrahigh-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UTHPLC/Q-TOF-MS) method for the detection of adulterated honey. Corn syrup, high-fructose corn syrup, inverted syrup, and rice syrup were used as honey adulterants; polysaccharides, difructose anhydrides, and 2-acetylfuran-3-glucopyranoside were used as detection markers. The presence of 10% sugar syrup in honey could be easily detected-in