FLUOROMETRIC ASSAY OF D-LACTATE
FLUOROMETRIC ASSAY OF D-LACTATE
复制标题
DOI：
10.1016/s0003-2697(05)80004-1
复制
发表时间：
1992-10-01
影响因子：
2.9
通讯作者：
THORNALLEY, PJ
中科院分区：
文献类型：
--
作者：
MCLELLAN, AC;PHILLIPS, SA;THORNALLEY, PJ
利用表皮葡萄球菌d-乳酸脱氢酶的终点酶测定法，建立了人类血液样品中n-乳酸的荧光测定方法。批内和批间方差系数分别为8.7% （n = 4）和16.6% （n = 4）。血中检出限为3.73 nmol/ml。该分析受到s -d-乳酸谷胱甘肽的轻微干扰，该物质也存在于血液样本中。正常健康人血中n-乳酸盐浓度（平均值±SE， nmol/ml）为11.0±1.2 (n = 7)；糖尿病患者20.0±1.3例（n = 55）（糖尿病显著增加，P < 0.01， Mann-Whitney U检验）。
A fluorimetric assay for n-lactate in human blood samples was developed using an endpoint enzymatic assay with d-lactate dehydrogenase from Staphylococcus epidermidis. The intrabatch and interbatch coefficients of variance were 8.7% (n = 4) and 16.6% (n = 4), respectively. The limit of detection in blood was 3.73 nmol/ml. The assay suffers minor interference from S-d-lactoylglutathione, which was also present in the blood samples. The concentration of n-lactate in blood was (mean ± SE, nmol/ml) normal healthy individuals, 11.0 ± 1.2 (n = 7); and diabetic patients, 20.0 ± 1.3 (n = 55) (a significant increase in diabetes mellitus; P < 0.01, Mann-Whitney U test).