Serum hepatocyte growth factor as a prognostic marker for stage II or III colorectal cancer patients
Serum hepatocyte growth factor as a prognostic marker for stage II or III colorectal cancer patients
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DOI：
10.1002/ijc.24554
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发表时间：
2009-10-01
影响因子：
6.4
通讯作者：
Kusunoki, Masato
中科院分区：
文献类型：
--
作者：
Toiyama, Yuji;Miki, Chikao;Kusunoki, Masato
我们之前报道过血清肝细胞生长因子（HGF）和癌胚抗原（CEA）的组合可用于选择具有侵袭性疾病的早期结直肠癌患者。本研究的目的是确定血清 HGF 是否可以为接受治疗性手术的患者提供独立于 CEA 的预后信息。血清样本采集自 184 名结直肠癌患者和 30 名对照者。采用逆转录聚合酶链反应检测结直肠癌细胞系中HGF的表达。通过酶联免疫吸附测定法测量 HGF 的血清和组织水平。将结直肠癌患者的血清 HGF 水平与健康对照进行比较，并回顾性评估血清 HGF 水平与临床病理结果和生存之间的关联。结直肠癌组织中HGF的表达显着高于非肿瘤组织。血清HGF水平与癌组织中HGF水平密切相关。患者血清HGF平均水平显着高于对照组，且肿瘤较大、淋巴结受累和远处转移的患者显着升高。根据受试者工作特征（ROC）分析，血清HGF水平升高可以预测患者患有较大的肿瘤、淋巴结和远处转移。血清 HGF 水平升高与生存不良显着相关，并且仅是 II 期或/和 III 期生存不良的独立危险因素。血清 HGF 水平升高与结直肠癌的发展、淋巴或远处侵袭表型以及生存显着相关，特别是在 II 期或 III 期患者中。 (C)2009年UICC
We previously reported that a combination of serum hepatocyte growth factor (HGF) and carcinoembryonic antigen (CEA) was useful for selecting early-stage colorectal cancer patients with aggressive disease. The aim of the present study was to determine whether serum HGF could provide CEA-independent prognostic information on patients undergoing surgery with curative intent. Serum samples were collected from 184 patients with colorectal cancer and 30 controls. Reverse-transcription polymerase chain reaction was used to detect HGF expression in colorectal cancer cell lines. Serum and tissue levels of HGF were measured by enzyme-linked immunosorbent assay. The serum HGF levels in colorectal cancer patients were compared with those in healthy controls, and we retrospectively assessed the association between serum HGF levels and clinicopathological findings and survival. Expression of HGF was significantly higher in colorectal cancer tissues compared with non-tumor tissues. The serum HGF levels were closely correlated with the HGF levels in cancer tissue. The mean serum HGF level in patients was significantly higher than that in controls, and significantly higher in patients with large tumor, lymph-node involvement and distant metastasis. According to the receiver operating characteristic (ROC) analysis, elevated serum HGF levels can predict patients with larger tumor, lymphnode and distant metastasis. Elevated serum HGF level demonstrated a significant association with poor survival, and was only an independent risk factor for poor survival in Stage II or/and III. Elevated serum HGF level is significantly associated with colorectal cancer development, lymph or distant invasive phenotypes and survival, especially in Stage II or III patients. (C) 2009 UICC