Both irradiated and bystander effects link with DNA repair capacity and the linear energy transfer
Both irradiated and bystander effects link with DNA repair capacity and the linear energy transfer
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辐射效应和旁观者效应都与 DNA 修复能力和线性能量转移有关
DOI：
10.1016/j.lfs.2019.03.013
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发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Shao Chunlin
中科院分区：
文献类型：
--
作者：
Tu Wenzhi;Dong Chen;Fu Jiamei;Pan Yan;Kobayashi Alisa;Furusawad Yoshiya;Konishi Teruaki;Shao Chunlin
目的与低线性能量转移（LET）辐射相比，高LET辐射诱导的DNA损伤更为复杂。本研究探讨辐射诱导的旁观者效应（里贝）是否依赖于LET。材料与方法中国仓鼠卵巢CHO-9细胞及其亚系EM-C11细胞（SSB修复缺陷）和XR-C1细胞（DSB修复缺陷）分别受到13、30和70 keV/μm的γ射线、α粒子和碳离子的照射。采用CCK-8、集落形成、微核（MN）、流式细胞术和western blot等方法分别检测辐射对细胞增殖、细胞死亡、DNA损伤、细胞周期分布和蛋白表达的影响。G2/M期阻滞及γ H2 AX蛋白表达。这些细胞损伤也依赖于DNA修复能力，并且XR-C1细胞对每种辐射最敏感。此外，当用从辐照的CHO-9细胞收集的条件培养基（CM）处理细胞时，EM-C11或XR-C1的旁观者细胞中的MN诱导和细胞死亡反应沿着辐照的LET而增加，EM-C11和XR-C1细胞比CHO-1细胞更容易产生旁观者损伤。细胞DNA修复能力和辐射的LET值不仅会深刻影响受照细胞的损伤程度，而且还会影响旁观者细胞的损伤程度。
AimsIn comparison with a low linear energy transfer (LET) radiation, a high-LET radiation induces more complex DNA damage. This study wonders whether radiation-induced bystander effect (RIBE) is dependent of LET.Materials and methodsChinese hamster ovary CHO-9 cells and its subline EM-C11 cells (SSB repair deficient) and XR-C1 cells (DSB repair deficient) were irradiated by γ-rays, α-particles, or carbon ions with different LETs of 13, 30 and 70 keV/μm. Cell proliferation, cell death, DNA damage, cell cycle distribution and some protein expressions were measured with the cell counting kit-8 (CCK-8), colony formation, micronuclei (MN), flow cytometry and western blot, respectively.Key findingsA series of cell responses were induced by these radiations in a LET-dependent manner, including proliferation inhibition, cell death, MN induction, G2/M phase arrest and the expression of γH2AX protein. These cell injuries were also depended on DNA repair capacity, and XR-C1 cells were the most sensitive to each radiation. Furthermore, when the cells were treated with the conditioned medium (CM) collected from irradiated CHO-9 cells, the MN induction and cell death response in the bystander cells of EM-C11 or XR-C1 increased along with LET of irradiation, and the bystander damage was easier to be induced in EM-C11 and XR-C1 cells than that in CHO-9 cells.SignificanceBoth cellular DNA repair capacity and the LET value of radiation could deeply influence damage extents of not only the irradiated cells but also the bystander cells.