Endogenous estrogen attenuates pulmonary artery vasoreactivity and acute hypoxic pulmonary vasoconstriction: the effects of sex and menstrual cycle
Endogenous estrogen attenuates pulmonary artery vasoreactivity and acute hypoxic pulmonary vasoconstriction: the effects of sex and menstrual cycle
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DOI：
10.1152/ajpendo.00201.2007
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发表时间：
2007-09-01
影响因子：
5.1
通讯作者：
Meldrum, Daniel R.
中科院分区：
文献类型：
--
作者：
Lahm, Tim;Patel, Ketan M.;Meldrum, Daniel R.
多种心血管疾病都存在性别差异。性激素已被证明可以介导肺动脉（PA）血管舒张。然而，由于性别和月经周期引起的生理性激素水平波动对 PA 血管反应性的影响尚未明确。我们假设性别和月经周期在常氧和缺氧条件下都会影响 PA 血管收缩。在发情前期雌性 (PF)、发情期和发情间期雌性 (E/DF) 以及雄性 (M) Sprague-Dawley 大鼠的分离 PA 环中测量等长力位移。用 80 mmol/l KCl 和 1 μmol/l 去氧肾上腺素刺激后，测量常氧条件下（用 95% O-2-5% CO2 的器官浴）的血管收缩反应。通过将气体改为95% N-2-5% CO2 来产生缺氧。与 E/DF 环相比，PF 的 PA 环对 KCl 的血管收缩反应减弱（75.58 +/-3.2% 对比 92.43 +/-4.24%，P < 0.01）。与 E/DF 相比，来自 M 的环还表现出减弱的 KCl 诱导的血管收缩（79.34 +/-3.2% 对比 92.43 +/-4.24%，P < 0.05）。与 E/DF 相比，PF 的 PA 环对去氧肾上腺素表现出减弱的血管收缩反应（59.61 +/-2.98% 对比 70.03 +/-4.61%，P < 0.05）。虽然缺氧期间 PF、E/DF 和 M 之间的最大 PA 血管舒张没有差异，但 PF 中 PA 中缺氧肺血管收缩的 II 期明显减弱（M 中为 64.10 +/-7.10% 对比 83.91 +/-5.97%，P < 0.05）。我们得出结论，性别和月经周期影响孤立 PA 环中的 PA 血管收缩。在常氧和缺氧条件下，即使循环雌激素水平的生理性增加也会减弱 PA 血管收缩。
Sex differences exist in a variety of cardiovascular disorders. Sex hormones have been shown to mediate pulmonary artery (PA) vasodilation. However, the effects of fluctuations in physiological sex hormone levels due to sex and menstrual cycle on PA vasoreactivity have not been clearly established yet. We hypothesized that sex and menstrual cycle affect PA vasoconstriction under both normoxic and hypoxic conditions. Isometric force displacement was measured in isolated PA rings from proestrus females (PF), estrus and diestrus females (E/DF), and male (M) Sprague-Dawley rats. The vasoconstrictor response under normoxic conditions (organ bath bubbled with 95% O-2-5% CO2) was measured after stimulation with 80 mmol/l KCl and 1 mu mol/l phenylephrine. Hypoxia was generated by changing the gas to 95% N-2-5% CO2. PA rings from PF demonstrated an attenuated vasoconstrictor response to KCl compared with rings from E/DF (75.58 +/-3.2% vs. 92.43 +/-4.24%, P < 0.01). Rings from M also exhibited attenuated KCl-induced vasoconstriction compared with E/DF (79.34 +/-3.2% vs. 92.43 +/-4.24%, P < 0.05). PA rings from PF exhibited an attenuated vasoconstrictor response to phenylephrine compared with E/DF (59.61 +/-2.98% vs. 70.03 +/-4.61%, P < 0.05). While the maximum PA vasodilation during hypoxia did not differ between PF, E/DF, and M, phase II of hypoxic pulmonary vasoconstriction was markedly diminished in the PA from PF (64.10 +/-7.10% vs. 83.91 +/-5.97% in M, P < 0.05). We conclude that sex and menstrual cycle affect PA vasoconstriction in isolated PA rings. Even physiological increases in circulating estrogen levels attenuate PA vasoconstriction under both normoxic and hypoxic conditions.