STEAROYL COENZYME-A DESATURASE ENZYME-ACTIVITY AND MESSENGER-RNA LEVELS ARE NOT DIFFERENT IN SUBCUTANEOUS ADIPOSE-TISSUE FROM ANGUS AND AMERICAN WAGYU STEERS
STEAROYL COENZYME-A DESATURASE ENZYME-ACTIVITY AND MESSENGER-RNA LEVELS ARE NOT DIFFERENT IN SUBCUTANEOUS ADIPOSE-TISSUE FROM ANGUS AND AMERICAN WAGYU STEERS
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DOI：
10.2527/1994.72102624x
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发表时间：
1994-10-01
影响因子：
3.3
通讯作者：
SMITH, SB
中科院分区：
文献类型：
--
作者：
CAMERON, PJ;ROGERS, M;SMITH, SB
我们提出，更大的硬脂酰辅酶A（CoA）去饱和酶的酶活性导致美国和牛脂肪组织中观察到的单不饱和脂肪酸升高。在饲喂至日本市场终点的安格斯（n = 5）和美国和牛（n = 5）皮下脂肪样品中测定硬脂酰CoA去饱和酶mRNA浓度和酶活性。使用大鼠肝硬脂酰CoA去饱和酶cDNA克隆来测量硬脂酰CoA去饱和酶mRNA的相对量。酶的活性和mRNA的浓度，通过激光密度测定的狭缝印迹放射自显影，没有显着差异，在这个阶段的增长，两个品种之间。这项调查表明，在这个成熟阶段，安格斯和美国和牛之间的脂肪酸组成的差异不能归因于硬脂酰CoA去饱和酶的酶活性的差异。
We proposed that greater stearoyl coenzyme A (CoA) desaturase enzyme activity caused the elevated monounsaturated fatty acids observed in American Wagyu adipose tissue. Stearoyl CoA desaturase mRNA concentrations and enzyme activities were measured in subcutaneous adipose samples from Angus (n = 5) and American Wagyu (n = 5), fed to the Japanese market end point. A rat liver stearoyl CoA desaturase cDNA clone was used to measure the relative amounts of stearoyl CoA desaturase mRNA. Enzyme activities and mRNA concentrations, as measured by laser densitometry of slot-blot autoradiograms, were not significantly different between the two breeds at this stage of growth. This investigation has demonstrated that, at this stage of maturity, differences in fatty acid composition between Angus and American Wagyu steers cannot be attributed to differences in stearoyl CoA desaturase enzyme activity.