In vivo MR imaging of plaque disruption and thrombus formation in an atherosclerotic rabbit model
In vivo MR imaging of plaque disruption and thrombus formation in an atherosclerotic rabbit model
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DOI：
10.1007/s10554-011-9854-x
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发表时间：
2012-03
期刊：
影响因子：
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通讯作者：
Xiaohai Ma;Quanming Zhao;Lei Zhao;Jian-feng Shang;T. Feng;Conghe Zeng;Zhao-qi Zhang
中科院分区：
文献类型：
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作者：
Xiaohai Ma;Quanming Zhao;Lei Zhao;Jian-feng Shang;T. Feng;Conghe Zeng;Zhao-qi Zhang
我们的目的是介绍一种动脉粥样硬化家兔模型，用于诱导家兔动脉粥样硬化病变，并在体内用3 T高分辨率磁共振成像验证血栓形成后，触发斑块破裂的模型。将20只雄性新西兰白色兔随机分为实验组（n = 16）和对照组（n = 4）。实验组家兔喂饲高胆固醇饲料2周后，用血管内球囊损伤主动脉壁。在总共16周的间歇性胆固醇喂养后，用拉塞尔蝰蛇毒液和组胺进行药理学触发。所有动物均行触发前和触发后MR检查，包括TOF、T1 WI、T2 WI和增强后T1 WI。所有家兔均行安乐死，取主动脉大体解剖和组织学标本。对MRI图像进行分析，并与组织学结果进行比较。与对照组兔相比，实验组兔主动脉在预触发MR图像上显示血管壁增厚、管腔变窄、血管壁强化程度增加。14只家兔在触发后存活，其中8只发生血栓形成（58.1%）。对照组无血栓形成。TOF、T1 WI、T2 WI及增强后T1 WI多序列MR对血栓的检出率为87.1%（27/31）。血栓长度（r = 0.94，P < 0.01）和血栓位置（r = 0.85，P < 0.01）与病理组织学检查结果相关。研究表明，MR可靠地确定了动脉粥样硬化兔模型中的斑块破裂和血栓形成。
Our aim is to introduce an atherosclerotic rabbit model for inducing atherosclerosis lesions in rabbits, and to validate the model in vivo with 3T high resolution magnetic resonance imaging of the thrombosis followed a pharmacologically triggered plaque disruption. Twenty male New Zealand White rabbits were randomly allocated into an experimental group (n = 16) and a control group (n = 4). The aortic wall injuries were induced by an intravascular balloon in the experimental group rabbits after feeding them with a high cholesterol diet for 2 weeks. The pharmacological triggering with Russell’s viper venom and histamine was performed after totally 16 weeks of intermittent cholesterol feeding. All of the animals underwent both the pre-trigger and post-trigger MR examinations including TOF, T1WI, T2WI and post contrast T1WI. Euthanasia was performed in all rabbits; gross anatomy and histological specimen of aorta were obtained. MR images were analyzed and compared with histological results. Compared with the control group rabbits, the aorta of the experimental group rabbits in the pre-triggered MR images showed an increased vessel wall thickening, luminal narrowing, and vessel wall enhancement. Fourteen rabbits survived the triggering, and 8 of them developed thrombosis (58.1%). No thrombus was found in the control group. The accuracy of the multi-sequences MR including TOF, T1WI, T2WI and post contrast T1WI was 87.1% (27/31) for detecting thrombus. MR data significantly correlated with the histopathology data for both thrombus length (r = 0.94,P< 0.01) and thrombus location (r = 0.85,P< 0.01), respectively. The study demonstrated that MR reliably determined the plaque disruption and thrombus formation in the atherosclerotic rabbit model.