Magnetic resonance microscopy of the C57BL mouse brain
Magnetic resonance microscopy of the C57BL mouse brain
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DOI：
10.1006/nimg.2000.0567
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发表时间：
2000-06-01
期刊：
影响因子：
5.7
通讯作者：
Johnson, GA
中科院分区：
文献类型：
--
作者：
Benveniste, H;Kim, K;Johnson, GA
随着基因技术的快速发展，转基因、靶向和化学诱导的小鼠突变不断产生。这些研究的主要目标是了解和表征基因型对解剖学、生理学和行为的影响，并最终了解基因型在疾病发展中的作用。对具有高空间分辨率潜力的成像技术的需求正在上升，因为这种成像工具将加快遗传改变小鼠的解剖表型。磁共振显微镜（MRM）是一种非侵入性的，固有的三维（3D）成像技术，能够可视化几个解剖结构的小老鼠。MRM的3D性质还允许解释子结构之间的复杂空间关系，这在解剖学表型分析时很重要。本文的目的是系统地描述三个主要的脑区在C57 BL/6 J小鼠在显微解剖空间分辨率范围内使用体外MRM。我们探索不同的MR对比参数，体素大小，和信噪比，以最好地表征C57 BL/6 J小鼠脑微结构的MRM。此外，我们将所有的MRM图像与Nissl染色的脑切片进行比较。主要发现如下：T2* MR图像显示了小鼠大脑中的几个大体解剖区域，但没有显示海马内的子区域。另一方面，扩散质子染色上级T2* MR图像，并描绘了海马内的许多亚区。最后，对比增强有助于在T2* MR图像上显示海马解剖结构。这项研究的结果是我们中心正在进行的一项计划的一部分，该计划的重点是通过MRM创建一个完整的C57 BL/6 J小鼠解剖3D图像数据库。(C)北京大学出版社.
With the rapid progression in gene technologies, transgenic, targeted, and chemically induced mutations in mice are continually created. The major goal of these studies is to understand and characterize the effects of genotype on anatomy, physiology, and behavior and ultimately the role of genotype in development of disease. The demand for imaging techniques with high spatial resolution potential is rising because such imaging tools would expedite anatomical phenotyping in the genetically altered mice. Magnetic resonance microscopy (MRM) is a noninvasive, inherently three-dimensional (3D) imaging technique capable of visualizing several anatomical structures in the small mouse. The 3D nature of MRM also allows for interpretation of complex spatial relationships between substructures, which is important when phenotyping anatomically. The goal of this paper is to systematically describe three major brain regions in the C57BL/6J mouse at microanatomical spatial resolution ranges using in vitro MRM. We explore different MR contrast parameters, voxel sizes, and signal-to-noise ratios to best characterize C57BL/6J mouse brain microstructure by MRM. Further, we compare all MRM images with Nissl-stained brain sections. Major findings were as follows: T2* MR images visualized several gross anatomical regions in the mouse brain but not, for example, subregions within the hippocampus. Diffusion proton stains on the other hand were superior to T2* MR images and delineated many subregions within the hippocampus proper. Finally, contrast enhancement facilitated visualization of hippocampal anatomy on the T2* MR images. The results of this study are part of an ongoing initiative at our Center focused on creating a complete C57BL/6J mouse anatomical 3D image database by MRM. (C) 2000 Academic Press.