CALCIUM-INDUCED ASYMMETRICAL BEATING OF TRITON-DEMEMBRANATED SEA-URCHIN SPERM FLAGELLA
CALCIUM-INDUCED ASYMMETRICAL BEATING OF TRITON-DEMEMBRANATED SEA-URCHIN SPERM FLAGELLA
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DOI：
10.1083/jcb.82.2.401
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发表时间：
1979-01-01
影响因子：
7.8
通讯作者：
BROKAW, CJ
中科院分区：
文献类型：
--
作者：
BROKAW, CJ
不对称弯曲波可以通过在高Ca 2+浓度下重新激活去膜海胆精子来获得。移动电影闪光灯摄影显示，不对称鞭毛弯曲波与提前终止的增长的弯曲在1个方向（反向弯曲），而在相反的方向（主弯曲）的弯曲增长1个完整的节拍周期，并与不等的增长率的主要和反向弯曲。这两种不对称成分的相对比例是高度可变的。主弯曲中增加的角度由反向弯曲中减小的角度补偿，使得平均弯曲角度没有变化;波长和拍频也与不对称度无关。这些新的信息仍然不足以确定Ca 2+诱导的不对称性的特定机制。当发育中的弯曲在相同方向上的新弯曲生长开始之前停止生长时，需要修改鞭毛远端部分中的小管之间的滑动。这种变形可以描述为同步滑动叠加在与传播弯曲波相关的异时滑动上。同步滑动在高度不对称的鞭毛中特别明显，但可能不是不对称的原因。异时滑动的控制似乎不受同步滑动的叠加的影响。
Asymmetrical bending waves can be obtained by reactivating demembranated sea urchin spermatozoa at high Ca2+ concentrations. Moving-film flash photography shows that asymmetrical flagellar bending waves are associated with premature termination of the growth of the bends in 1 direction (the reverse bends) while the bends in the opposite direction (the principal bends) grow for 1 full beat cycle, and with unequal rates of growth of principal and reverse bends. The relative proportions of these 2 components of asymmetry are highly variable. The increased angle in the principal bend is compensated by a decreased angle in the reverse bend, so that there is no change in mean bend angle; the wavelength and beat frequency are also independent of the degree of asymmetry. This new information is still insufficient to identify a particular mechanism for Ca2+-induced asymmetry. When a developing bend stops growing before initiation of growth of a new bend in the same direction, a modification of the sliding between tubules in the distal portion of the flagellum is required. This modification can be described as a superposition of synchronous sliding on the metachronous sliding associated with propagating bending waves. Synchronous sliding is particularly evident in highly asymmetrical flagella, but is probably not the cause of asymmetry. The control of metachronous sliding appears to be unaffected by the superposition of synchronous sliding.