The respiratory system of Pseudomonas putida: participation of cytochromes in electron transport.
The respiratory system of Pseudomonas putida: participation of cytochromes in electron transport.
复制标题
恶臭假单胞菌的呼吸系统：细胞色素参与电子传递。
DOI：
10.1016/0003-9861(81)90279-4
复制
发表时间：
1981
影响因子：
3.9
通讯作者：
Peterson,JA
中科院分区：
文献类型：
--
作者：
Sweet,WJ;Peterson,JA
采用电子给体还原性烟酰胺腺嘌呤二核苷酸（NADH）和琥珀酸盐，氧化还原染料2,6-二氯苯酚和N，N,N ',N ' -四甲基-对苯二胺测定了恶臭假单胞菌呼吸颗粒物的氧利用率。NADH和琥珀酸盐产生的最大速率在对数相和固定相细胞中都是相似的，但在固定相细胞中使用染料测量的速率要高得多。在这两种情况下，氰化物和叠氮化物都是非常有效的染料氧化抑制剂，但它们只在对数相颗粒中产生部分抑制NADH和琥珀酸盐氧化，而在固定相中没有作用。在不同的电子供体和抑制剂产生的几个还原水平上的细胞色素的光谱检查表明，大多数被染料还原的细胞色素位于氰化物敏感的电子传递途径上。这些发现支持了p。嘌呤在固定相产生一个电子传递系统，涉及细胞色素水平上的分支。对于对数相颗粒中的四种底物，CO对氧利用的抑制几乎完全完成。在固定相中对染料氧化也有一定的抑制作用，但对NADH和琥珀酸盐氧化没有影响。CO抑制解除的光化学作用谱显示，对数相颗粒中的NADH和琥珀酸盐氧化可能涉及细胞染色。两种类型的颗粒对染料的氧化似乎也涉及细胞色素，并且也表明了非ord型细胞色素参与的可能性。
Rates of oxygen utilization byPseudomonas putidarespiratory particles were measured using the electron donors, reduced nicotinamide adenine dinucleotide (NADH) and succinate, and the oxidation-reduction dyes, 2,6-dichlorophenolindophenol andN,N,N′,N′-tetramethyl-p-phenylenediamine. The maximal rates produced by NADH and succinate were similar for particles from either log- or stationary-phase cells, but rates measured using the dyes were much higher in stationary-phase particles. Cyanide and azide were very effective inhibitors of dye oxidation in both cases, but they produced only partial inhibition of NADH and succinate oxidation in log-phase particles and had no effect in the stationary phase. Spectral examination of the cytochromes at several levels of reduction produced by the various electron donors and inhibitors indicated that most of the cytochromes that were reduced by the dyes lie on a cyanide sensitive pathway of electron transport. These findings support the hypothesis thatP. putidaproduces an electron transport system in the stationary phase which involves branching at the level of the cytochromes.Inhibition of oxygen utilization by CO was nearly complete for all four substrates in logphase particles. Inhibition was also reasonably effective for dye oxidation in the stationary phase, but there was no effect on NADH or succinate oxidation. Photochemical action spectra of the relief of CO inhibition revealed that NADH and succinate oxidation in log-phase particles probably involves cytochromeo. Oxidation of the dyes by either type of particles also appeared to involve cytochromeo, and the possibility of the participation of ana- ord-type cytochrome was also indicated.