NUCLEOTIDE-SEQUENCE OF A BOVINE CLONE ENCODING THE ANGIOGENIC PROTEIN, BASIC FIBROBLAST GROWTH-FACTOR
NUCLEOTIDE-SEQUENCE OF A BOVINE CLONE ENCODING THE ANGIOGENIC PROTEIN, BASIC FIBROBLAST GROWTH-FACTOR
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DOI：
10.1126/science.2425435
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发表时间：
1986-08-01
期刊：
影响因子：
56.9
通讯作者：
FIDDES, JC
中科院分区：
文献类型：
--
作者：
ABRAHAM, JA;MERGIA, A;FIDDES, JC
碱性和酸性成纤维细胞生长因子（FGF）在体外是毛细血管内皮细胞的有效有丝分裂原，在体内刺激血管生成，并且可以参与组织修复。牛碱性FGF的寡核苷酸探针的设计从牛酸性FGF的氨基末端外显子的核苷酸序列，考虑到55%的氨基酸序列之间的同源性的两个因素。用此寡核苷酸探针从牛垂体中分离到碱性成纤维细胞生长因子的全长互补DNA。牛下丘脑中的碱性FGF被证明由单个5.0-β-淀粉酶信使RNA编码;在人肝癌细胞系中，存在4.6-β-淀粉酶和2.2-β-淀粉酶碱性FGF信使RNA。这两种生长因子似乎都是用短的氨基末端延伸合成的，这在氨基酸序列已确定的分离形式中没有发现。碱性和酸性FGF都没有经典的信号肽。
Basic and acidic fibroblast growth factors (FGF''s) are potent mitogens for capillary endothelial cells in vitro, stimulate angiogenesis in vivo, and may participate in tissue repair. An oligonucleotide probe for bovine basic FGF was designed from the nucleotide sequence of the amino-terminal exon of bovine acidic FGF, taking into account the 55 percent amino acid sequence homology between the two factors. With this oligonucleotide probe, a full length complementary DNA for basic FGF was isolated from bovine pituitary. Basic FGF in bovine hypothalamus was shown to be encoded by a single 5.0-kilobase messenger RNA; in a human hepatoma cell line, both 4.6- and 2.2-kilobase basic FGF messenger RNA''s were present. Both growth factors seem to be synthesized with short amino-terminal extensions that are not found on the isolated forms for which the amino acid sequences have been determined. Neither basic nor acidic FGF has a classic signal peptide.