Hormonal regulation of heme oxygenase induction in avian hepatocyte culture.
Hormonal regulation of heme oxygenase induction in avian hepatocyte culture.
复制标题
禽肝细胞培养物中血红素加氧酶诱导的激素调节。
DOI：
10.1016/0006-2952(85)90019-x
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发表时间：
1985
影响因子：
5.8
通讯作者：
Kappas,A
中科院分区：
文献类型：
--
作者：
Sardana,MK;Sassa,S;Kappas,A
在化学成分确定的培养基中培养的鸡胚肝细胞单层培养物中，研究了各种激素对血红素加氧酶诱导的影响。此外，胰岛素的培养细胞显着抑制的基础以及钴+诱导的血红素加氧酶的活性。用氢化可的松处理细胞也抑制了基础酶活性，而Co2+诱导的酶活性略有增强。相反，三碘甲腺原氨酸除了文化引起轻微增加的未诱导和诱导水平的酶。这种刺激作用的三碘甲腺原氨酸增强显着延长培养的细胞（48-96小时）在serumfree培养基。这些发现表明，血红素加氧酶的合成可以通过改变肝细胞的激素环境而发生实质性改变。此外，血红素加氧酶的诱导由钴+抑制胰高血糖素，二丁酰cAMP和茶碱的剂量依赖性方式，表明酶的诱导也可能是由cAMP水平的变化控制。
The effects of various hormones were examined on the induction of heme oxygenase in monolayer cultures in chick embryo hepatocytes maintained in a chemically defined medium. Addition of insulin to the cultured cells markedly suppressed the activity of basal as well as Co2+-induced heme oxygenase. Treatment of cells with hydrocortisone also suppressed the basal enzyme activity, while the Co2+-induced enzyme activity was enhanced slightly. In contrast, triiodothyronine addition to the culture caused a slight increase of both uninduced and induced levels of the enzyme. This stimulatory effect of triiodothyronine was enhanced significantly by prolonged incubation of cells (48–96 hr) in the serumfree medium. These findings indicate that heme oxygenase synthesis can be substantially altered by changing the hormonal environment of the hepatocytes. Furthermore, the induction of heme oxygenase by Co2+was inhibited by glucagon, dibutyryl cAMP and theophylline in a dose-dependent manner, suggesting that the enzyme induction may also be controlled by changes in cAMP levels.