Visualizing genomes with Oligopaint FISH probes.
Visualizing genomes with Oligopaint FISH probes.
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DOI：
10.1002/0471142727.mb1423s105
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发表时间：
2014-01-06
影响因子：
--
通讯作者：
Wu, Chao-ting
中科院分区：
文献类型：
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作者：
Beliveau, Brian J;Apostolopoulos, Nicholas;Wu, Chao-ting
Oligopaint探针是荧光标记的单链DNA寡核苷酸，可用于可视化大小从几十千碱基到许多兆碱基的基因组区域。本单元详细介绍了寡聚涂料探针如何使用基本的分子生物学技术合成，并提供了FISH， 3D-FISH和样品制备的协议。
Oligopaint probes are fluorescently-labeled, single-stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many megabases. This unit details how Oligopaint probes can be synthesized using basic molecular biological techniques as well as provides protocols for FISH, 3D-FISH, and sample preparation.