Direct acoustic profiling of DNA hybridisation using HSV type 1 viral sequences.
Direct acoustic profiling of DNA hybridisation using HSV type 1 viral sequences.
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DOI：
10.1039/b711850c
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
Y. Uludağ;Xin Li;H. Coleman;S. Efstathiou;M. Cooper
中科院分区：
文献类型：
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作者：
Y. Uludağ;Xin Li;H. Coleman;S. Efstathiou;M. Cooper
我们描述了通过一种新型的，高灵敏度的声学生物传感器检测的特异性，单纯疱疹病毒（HSV）1型的保守DNA序列。对平面和聚合羧酸和生物素呈递表面化学的重复测定使得能够对测定特异性和灵敏度进行统计学比较，并评价测定Z因子评分。使用NeutrAvidin捕获进行三分钟杂交以增强信号，可以检测5.2 × 10（-11）M浓度的HSV病毒核酸。
We describe the detection of specific, conserved DNA sequences of herpes simplex virus (HSV) type 1 by means of a novel, high sensitivity acoustic biosensor. Repeated assays on planar and polymeric carboxylic acid- and biotin-presenting surface chemistries enabled statistical comparison of assay specificity and sensitivity and evaluation of assay Z-factor scores. Using a three minute hybridisation with NeutrAvidin capture for signal enhancement, it was possible to detect HSV viral nucleic acids at 5.2 x 10(-11) M concentration.