COMPARATIVE STUDIES OF RABIES AND SINDBIS VIRUS-REPLICATION IN HUMAN NEURO-BLASTOMA (SYM-I) CELLS THAT CAN PRODUCE INTERFERON
COMPARATIVE STUDIES OF RABIES AND SINDBIS VIRUS-REPLICATION IN HUMAN NEURO-BLASTOMA (SYM-I) CELLS THAT CAN PRODUCE INTERFERON
复制标题
DOI：
10.1099/0022-1317-65-10-1645
复制
发表时间：
1984-01-01
影响因子：
3.8
通讯作者：
MATSUMOTO, S
中科院分区：
文献类型：
--
作者：
HONDA, Y;KAWAI, A;MATSUMOTO, S
狂犬病Hep-Flury病毒诱导人神经母细胞瘤(SYM-1)克隆细胞株K-104细胞释放大量干扰素，其抗病毒活性显著抑制狂犬病病毒复制。通过抗干扰素抗体的处理，证实了内源性干扰素的作用，从而增加了后代病毒的产量。第二代未稀释的K-104细胞的病毒产量远低于第一代，这是由于病毒接种物中的干扰素在病毒吸附的短时间内与细胞短暂接触而启动的抗病毒状态。当M.O.I。[感染的多样性]与未稀释的第三代相比相对较低，接种物中存在的干扰素的作用得到增强，大多数受感染的细胞存活下来，但被证明处于持续感染状态。缺陷干扰(DI)颗粒在这3代未稀释的传代中没有快速积累。用Sindbis病毒感染K-104细胞，在未稀释的12代中，其内源性干扰素系统未被激活。在第12代，由于DI颗粒的产生和积累，产量开始下降。
K-104 cells, a cloned cell line derived from a human neuroblastoma (SYM-1), were induced by rabies HEP-Flury virus to release large amounts of interferon, and the resulting antiviral activity significantly suppressed the rabies virus replication. The role of endogenous interferon was confirmed by treatment with anti-interferon antibody which increased the yield of progeny virus. The virus yield in the second undiluted passage though K-104 cells was much less than that in the first passage, because of the antiviral state initiated by brief contact of interferon present in the virus inoculum with cells during the short period of virus adsorption. When the m.o.i. [multiplicity of infection] was relatively low as in the third undiluted passage, the effect of interferon present in the inoculum was enhanced and most of the infected cells survived but were shown to be in a state of persistent infection. Defective interfering (DI) particles did not accumulate rapidly during these 3 undiluted passages. When Sindbis virus was used for infection, the endogenous interferon system of K-104 cells was not activated during 12 undiluted passages. On the 12th passage, the yield began to decline due to the generation and accumulation of DI particles.