Comprehensive evaluation of differential long non-coding RNA and gene expression in patients with cartilaginous endplate degeneration of cervical vertebra.
Comprehensive evaluation of differential long non-coding RNA and gene expression in patients with cartilaginous endplate degeneration of cervical vertebra.
复制标题
颈椎软骨终板退变患者差异性长非编码RNA及基因表达的综合评价
DOI：
10.3892/etm.2020.9390
复制
发表时间：
2020-12
影响因子：
2.7
通讯作者：
Cheng X
中科院分区：
文献类型：
--
作者：
Yuan J;Jia J;Wu T;Liu X;Hu S;Zhang J;Ding R;Pang C;Cheng X
长链非编码RNA（lncRNA）是基因表达的重要调控因子，但其在颈椎软骨终板退变（CED）中的作用目前尚不清楚。本研究旨在研究lncRNA的表达水平，并分析其在颈椎骨折和颈椎病患者颈椎CED中的潜在功能。应用人竞争性内源性RNA（ceRNA）芯片分析颈椎骨折和颈椎病患者行颈前路椎间盘切除融合术后CE标本中lncRNA和mRNA的表达水平。使用基因本体论（GO）和京都基因和基因组百科全书（KEGG）途径分析鉴定并功能分析差异表达的lncRNA（DEL）或差异表达的基因（DEG）。基于DEL和DEG构建lncRNA-microRNA（miRNA）-mRNA ceRNA调控网络，并使用Cytoscape 3.7.2软件可视化ceRNA网络。总体而言，在CED和健康CE样本中使用逆转录定量PCR鉴定出一种下调的mRNA、一种上调的miRNA和五种下游调节的lncRNA。共鉴定出369个lncRNA和246个mRNA在CE中差异表达。GO和KEGG分析表明，大多数GO和KEGG富集与CED相关。此外，还建立了一个ceRNA网络，包括168个推定的miRNA反应元件，189个上调和37个下调的lncRNA，47个上调和10个下调的DEG。本研究分析了DEG在ceRNA网络中的功能，并筛选出与DEG功能富集分析相同的项目。这些结果为进一步了解ceRNA介导的基因调控在颈椎病中的作用提供了新的视角，也为进一步研究lncRNA在颈椎病中的作用提供了新的理论基础。然而，需要进一步的实验来验证本研究的结果。
Long non-coding RNAs (lncRNAs) are emerging as key regulators in gene expression; however, little is currently known regarding their role in cartilaginous endplate (CE) degeneration (CED) of cervical vertebra. The present study aimed to investigate the expression levels of lncRNAs and analyze their potential functions in CED of cervical vertebra in patients with cervical fracture and cervical spondylosis. Human competitive endogenous RNA (ceRNA) array was used to analyze lncRNA and mRNA expression levels in CE samples from patients with cervical fracture and cervical spondylosis, who received anterior cervical discectomy and fusion. Differentially expressed lncRNAs (DELs) or differentially expressed genes (DEGs) were identified and functionally analyzed, using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. An lncRNA-microRNA(miRNA)-mRNA ceRNA regulatory network was constructed based on the DELs and DEGs, and the ceRNA network was visualized using Cytoscape 3.7.2 software. In total, one downregulated mRNA, one upregulated miRNA and five downstream regulated lncRNAs were identified using reverse transcription-quantitative PCR in CED and healthy CE samples. A total of 369 lncRNAs and 246 mRNAs were identified as differentially expressed in CE. The GO and KEGG analyses demonstrated that the majority of GO and KEGG enrichments were associated with CED. Furthermore, a ceRNA network was established, including 168 putative miRNA response elements, 189 upregulated and 37 downregulated lncRNAs and 47 upregulated and 10dow regulated DEGs. The present study analyzed the function of DEGs in the ceRNA network and filtered out the same items as in DEG-function enrichment analysis. These results provide a new perspective for an improved understanding of ceRNA-mediated gene regulation in cervical spondylosis, and provide a novel theoretical basis for further studies on the function of lncRNA in cervical spondylosis. However, further experiments are required to validate the results of the present study.
登录
查看更多内容
影响因子：
24.8
作者：
Gershman SJ;Daw ND
通讯作者：
Daw ND
影响因子：
4
作者：
Guo, Ling;Zhu, Ye;Cui, Hujun
通讯作者：
Cui, Hujun
DOI：
10.12659/msm.908219
发表时间：
2018-07-30
期刊：
Medical science monitor : international medical journal of experimental and clinical research
影响因子：
--
作者：
Chen Y;Wang X;Yang M;Ruan W;Wei W;Gu D;Wang J;Guo X;Guo L;Yuan Y
通讯作者：
Yuan Y
影响因子：
3.9
作者：
Hirose, Naoto;Okamoto, Yuki;Tanimoto, Kotaro
通讯作者：
Tanimoto, Kotaro
Opg 缺失导致小鼠腰椎间盘新血管形成和炎症细胞因子表达增加
影响因子：
3
作者：
Li, Xiao-Feng;Xue, Chun-Chun;Shu, Bing
通讯作者：
Shu, Bing