Detection of Borrelia burgdorferi DNA in tick feces provides evidence for organism shedding during vector feeding.
Detection of Borrelia burgdorferi DNA in tick feces provides evidence for organism shedding during vector feeding.
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在蜱虫粪便中检测到伯氏疏螺旋体 DNA 为媒介喂养期间生物体脱落提供了证据。
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
R. Gilmore
中科院分区：
文献类型：
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作者：
T. Patton;G. Dietrich;R. Gilmore
莱姆病的病原体伯氏疏螺旋体通过硬蜱传播给易感宿主。蜱虫叮咬。然而，B.伯氏螺旋体通过排泄物途径从蜱虫身上脱落。在本研究中，B.感染伯氏菌的蜱被喂食小鼠，同时被胶囊限制在皮肤的特定区域。在喂食期间和之后，收集蜱粪便，并置于Barbour-Stoenner-Kelley（BSK）-II培养基中进行培养，并置于无菌水中进行聚合酶链反应（PCR）分析。尽管所有受试样本均为B培养阳性。除了一个来自感染蜱的粪便DNA样品外，所有来自感染蜱的粪便DNA样品均为PCR阳性。这些结果表明，B.伯氏螺旋体在排便期间从喂食蜱中脱落，并且表明螺旋体一旦暴露于外部环境就不能保持活力。这一发现对研究人员解释B有重要的影响。在进行蜱传播实验时，获得了伯氏特异性PCR结果。
Borrelia burgdorferi, the bacterium that causes Lyme disease, is transmitted to a susceptible host by Ixodes spp. tick bites. However, there is uncertainty whether B. burgdorferi are shed from ticks by the fecal route. In this study, B. burgdorferi-infected ticks were fed on mice while confined to a certain area of the skin by a capsule. During and after feeding, tick feces were collected and placed in Barbour-Stoenner-Kelley (BSK)-II media for cultivation and in sterile water for polymerase chain reaction (PCR) analysis. Although none of the tested samples were culture positive for B. burgdorferi, all but one of the fecal DNA samples from infected ticks were PCR positive. These results indicated that B. burgdorferi were shed from feeding ticks during defecation and suggest that the spirochetes did not remain viable once exposed to the outside environment. This finding has important ramifications for investigators interpreting B. burgdorferi-specific PCR results when conducting tick transmission experiments.
伯氏疏螺旋体的许多面孔。
DOI：
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发表时间：
2000
期刊：
Journal of molecular microbiology and biotechnology.
影响因子：
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作者：
Seshu,J;Skare,JT
通讯作者：
Skare,JT