A JAK-STAT pathway target gene encoding a single WAP domain (SWD)-containing protein from Litopenaeus vannamei
A JAK-STAT pathway target gene encoding a single WAP domain (SWD)-containing protein from Litopenaeus vannamei
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JAK-STAT 通路靶基因编码来自凡纳滨对虾的包含单个 WAP 结构域 (SWD) 的蛋白质
DOI：
10.1016/j.fsi.2019.04.046
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发表时间：
2019
影响因子：
4.7
通讯作者：
Xu Xiaopeng
中科院分区：
文献类型：
--
作者：
Yang Linwei;Luo Mengting;He Jianhui;Zuo Hongliang;Weng Shaoping;He Jianguo;Xu Xiaopeng
在虾中，JAK-STAT途径基本上涉及抗病毒和抗菌反应。然而，迄今为止，关于JAK-STAT信号通路的调控靶基因的报道很少。本研究从凡纳滨对虾（Litopenaeus vannamei）中鉴定出一个新的含WAP结构域的肽（LvSWD 4），预测其启动子含有多个STAT结合DNA基序。体外过表达JAK-STAT途径的STAT、JAK和Domeless 3个组分可显著增强LvSWD 4启动子的转录活性，体内沉默STAT和JAK-STAT途径上游调控因子IRF可下调LvSWD 4的表达，提示LvSWD 4可能是JAK-STAT途径的靶基因。感染革兰氏阴性菌、革兰氏阳性菌、真菌和病毒后，LvSWD 4的表达量显著增加，LvSWD 4的沉默增加了对虾对病毒的易感性。体外实验还表明，重组LvSWD 4蛋白对革兰氏阴性菌V具有明显的抑制活性。parahaemolyticusandE.大肠杆菌和革兰氏阳性菌。aureusandB. LvSWD 4基因的沉默显著影响了多种免疫功能基因的表达，降低了血细胞的吞噬活性。提示LvSWD 4作为STAT的靶基因，在对虾免疫过程中起着重要作用，可能是对虾JAK-STAT途径免疫功能的部分机制。
In shrimp, the JAK-STAT pathway is essentially implicated in both antiviral and antibacterial responses. However, few regulatory target genes of the JAK-STAT pathway in shrimp have been reported so far. In this study, a novel single WAP domain-containing peptide (LvSWD4) was identified from Pacific white shrimpLitopenaeus vannamei.The promoter of LvSWD4 was predicted to harbor multiple STAT-binding DNA motifs. Over-expression of the JAK-STAT pathway components STAT, JAK and Domelessin vitrosignificantly enhanced the transcriptional activity of the LvSWD4 promoter, andin vivosilencing of STAT and the the JAK-STAT pathway upstream regulator IRF down-regulated the expression of LvSWD4, suggesting that LvSWD4 could be a target gene of the JAK-STAT pathway. The expression of LvSWD4 was significantly increased after infection with Gram-negative and positive bacteria, fungi and virus, and silencing of LvSWD4 increased the susceptibility of shrimp toV. parahaemolyticusand WSSV infections.In vitroexperiments also demonstrated that the recombinant LvSWD4 protein had significant inhibitory activities against Gram negative bacteriaV. parahaemolyticusandE. coliand Gram positive bacteriaS. aureusandB. subtilis.Furthermore, silencing of LvSWD4in vivosignificantly affected expression of various immune functional genes and attenuated the phagocytic activity of hemocytes. These suggested that as a target gene of STAT, LvSWD4 was essentially implicated in shrimp immunity, which could constitute part of the mechanism underlying the immune function of the shrimp JAK-STAT pathway.