Monitoring of fungal biomass changed by Rhizopus oryzae in relation to amino acid and essential fatty acids profile in soybean meal, wheat and rice

Monitoring of fungal biomass changed by Rhizopus oryzae in relation to amino acid and essential fatty acids profile in soybean meal, wheat and rice
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DOI:
10.1080/19476337.2017.1359676
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发表时间:
2018-01-01
影响因子:
2.1
通讯作者:
Badiale-Furlong, Eliana
Badiale-Furlong, Eliana
中科院分区:
农林科学4区
文献类型:
--
作者:
Denardi-Souza, Taiana;Massarolo, Kelly Cristina;Badiale-Furlong, Eliana

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本研究评估了根霉对木质纤维素底物大豆、小麦和米糠中必需氨基酸和脂肪酸组成的影响。R.从巴西南部地区的农作物中分离到一株产自巴西南部地区的产自巴西南部地区的产自巴西南部每隔24 h分别用高效液相色谱-荧光检测器(HPLC-FL)和气相色谱-火焰离子化检测器(GC-FID)测定氨基酸和脂肪酸的含量。在大米、小麦和大豆麸皮生物质中发现的脂肪酸显示出3种酸的增加(分别为72%、64%和58%);然而,6水平不受影响。从营养角度来看,大豆和麦麸上的真菌生物量呈现出最完整的必需氨基酸谱,在培养96 h后,瓦尔(34%)、Leu(19%)、Lys(6%)和Phe(2%)增加。用R.农业工业副产品中的必需营养素会发生变化。本研究旨在评价根霉对大豆纤维素酶、淀粉酶和淀粉酶活性的影响。帕拉也是巴西南部地区根霉的生产者,有些具有遗传学特征,并在30 ℃下持续120小时的反应器中实现固态发酵。分别用HPLC-FL和CG-FID测定了24小时的氨基酸和葡萄糖酸的含量。在大豆的生物质中检测到的酸,其中三种酸分别为72%,64%和58%,但它们对六种酸的去除有影响。从营养学角度来看,大豆微生物量和大豆残体的氨基酸含量在96个月的栽培中呈上升趋势,其中瓦尔、赖氨酸和苯丙氨酸分别占34%、19%、6%和2%。在农业工业副产品的营养物质中栽培根霉。
This study evaluated the effect of Rhizopus oryzae, on essential amino acids and fatty acid profile on lignocellulosic substrates soybean, wheat and rice bran. R. oryzae was isolated from crop of Brazilian south region, genetically characterized and solid-state fermentation was carried on tray reactors during 120h/30 degrees C. The profile of amino acids and fatty acids was conducted every 24h by high performance liquid chromatography with fluorescence detector (HPLC-FL) and gas chromatography (GC)-flame ionization detector, respectively. Fatty acids found on rice, wheat and soybean bran biomass showed an increase in the 3 acids (72%, 64% and 58%, respectively); however, 6 level was not affected. Fungal biomass on soybean and wheat bran present the most complete essential amino acids profile from a nutritional point of view, increased in Val (34%), Leu (19%), Lys (6%) and Phe (2%) after 96h of cultivation. The cultivation with R. oryzae triggers changes on essential nutrients of the agro-industrial by-products. RESUMENEl presente estudio se oriento a evaluar el efecto provocado por Rhizopus oryzae en el perfil de aminoacidos y acidos grasos esenciales presentes en los sustratos lignocelulosicos de la soya, el trigo y el salvado de arroz. Para ello se aislo el Rhizopus oryzae de una cosecha procedente de una region del sur de Brasil, sometiendolo a una caracterizacion genetica, y se realizo la fermentacion en estado solido con reactores de bandeja durante 120 horas a 30 degrees C. Cada 24 horas se determino el perfil de aminoacidos y de acidos grasos empleando HPLC-FL y CG-FID, respectivamente. Los acidos grasos detectados en la biomasa de arroz, trigo y salvado de soya mostraron un aumento de acidos 3 (72%, 64% y 58%, respectivamente), sin que se viera afectado el nivel de los 6. Desde una perspectiva nutricional, se constata que la biomasa micotica de la soya y el salvado de trigo presenta el perfil mas completo de aminoacidos esenciales, registrandose incrementos en Val (34%), Leu (19%), Lys (6%) y Phe (2%) despues de 96 horas de cultivo. El cultivo con Rhizopus oryzae provoca alteraciones en los nutrientes esenciales de los subproductos agroindustriales.