Ca2+ and AtCaM3 are involved in the expression of heat shock protein gene in Arabidopsis
Ca2+ and AtCaM3 are involved in the expression of heat shock protein gene in Arabidopsis
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DOI：
10.1111/j.1365-3040.2005.01365.x
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发表时间：
2005-10-01
影响因子：
7.3
通讯作者：
Zhou, RG
中科院分区：
文献类型：
--
作者：
Liu, HT;Sun, DY;Zhou, RG
研究了钙和不同钙调蛋白亚型 (Ca2+-CaM) 在拟南芥 (Arabidopsis thaliana) 热休克 (HS) 信号转导中的参与。使用具有AtHsp18.2启动子/GUS融合基因的转基因拟南芥植物，发现添加CaCl2上调β-葡萄糖醛酸酶（GUS）活性水平，并通过钙离子螯合剂EGTA、钙离子通道阻滞剂LaCl3和维拉帕米或CaM拮抗剂下调β-葡萄糖醛酸酶（GUS）活性水平 N-(6-氨基己基)-5-氯-1-萘磺酰胺 (W7)、氯丙嗪 (CPZ) 和三氟拉嗪 (TFP)。 CaCl2不仅增加了HS后的GUS活性，而且在非HS条件下也上调了GUS活性。这些结果为 Ca2+-CaM 信号系统参与 HSP 基因表达提供了额外的支持。拟南芥中 9 个 CaM 基因 (AtCaM1-9) 的表达在 37 摄氏度下受到 HS 的差异调节。AtCaM3 和 AtCaM7 基因的表达在 HS 期间增加。 AtCaM3、AtCaM7 和 hsp18.2 基因的时间表达表明，AtCaM3 表达的上调发生早于 AtCaM7 或 hsp18.2。
The involvement of calcium and different calmodulin isoforms (Ca2+-CaM) in heat shock (HS) signal transduction in Arabidopsis (Arabidopsis thaliana) was investigated. Using transgenic Arabidopsis plants which have the AtHsp18.2 promoter/GUS fusion gene, it was found that the level of beta-glucuronidase (GUS) activity was up-regulated by the addition of CaCl2 and down-regulated by the calcium ion chelator EGTA, the calcium ion channel blockers LaCl3 and verapamil, or the CaM antagonists N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W7), chlorpromazine (CPZ) and trifluoperazine (TFP). CaCl2 not only increased the GUS activity after HS, but also up-regulated the GUS activity under non-HS conditions. These results provide additional support for the involvement of the Ca2+-CaM signalling system in HSP gene expression. The expression of nine CaM genes (AtCaM1-9) from Arabidopsis was differentially regulated by HS at 37 degrees C. The expression of AtCaM3 and AtCaM7 genes increased during HS. The temporal expression of the AtCaM3, AtCaM7 and hsp18.2 genes demonstrated that up-regulation of AtCaM3 expression occurred earlier than that of AtCaM7 or hsp18.2.