Inhibition of colon adenocarcinoma cell proliferation by flavonols is linked to a G2/M cell cycle block and reduction in cyclin D1 expression
Inhibition of colon adenocarcinoma cell proliferation by flavonols is linked to a G2/M cell cycle block and reduction in cyclin D1 expression
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DOI：
10.1016/j.foodchem.2011.07.033
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发表时间：
2012-02-01
期刊：
影响因子：
8.8
通讯作者：
Spencer, Jeremy P. E.
中科院分区：
文献类型：
--
作者：
Gomez-Alonso, Sergio;Collins, Vanessa J.;Spencer, Jeremy P. E.
有人提出适量饮用红酒可以降低结直肠癌的发病率。其抗癌作用部分是通过多酚对结肠癌上皮细胞的作用介导的。我们发现，不含花青素的红酒酚提取物（50μg/ml）（去除花青素是为了反映多酚对大肠的生物利用度）和主要的红酒黄酮醇槲皮素、杨梅素、落叶树苷和注射器亭（50μM）能够抑制结直肠上皮腺癌细胞的增殖。这种抗增殖活性部分是由黄酮醇的直接细胞毒性作用及其在 G2/M 期引起显着细胞周期阻断的能力介导的。在大多数情况下，黄酮醇诱导的抗增殖作用先于环氧合酶-2 (COX-2) 和细胞周期蛋白 D1 表达的强烈且显着的降低。这些结果表明，观察到的结肠癌与适量红酒摄入之间的负相关可能部分是由红酒黄酮醇对癌细胞生长的作用介导的。 (C) 2011 Elsevier Ltd. 保留所有权利。
The moderate consumption of red wine has been proposed to reduce the incidence of colorectal cancer. Its anti-cancer action my in part be mediated by the actions of polyphenols on colon cancer epithelial cells. We show that a red wine phenolic (50 mu g/ml) extract devoid of anthocyanins (removed to reflect polyphenol bioavailability to the large intestine) and the major red wine flavonols quercetin, myricetin, laricitrin and syringetin (50 mu M) are capable of inhibiting the proliferation of colorectal epithelial adenocarcinoma cells. This anti-proliferative activity was partly mediated by the direct cytotoxic actions of flavonols and also their ability to cause a significant cell cycle blockage in G2/M. The anti-proliferative effects induced by flavonols were preceded, in most cases, by a strong and significant reduction of cyclooxygenase-2 (COX-2) and cyclin D1 expression. These results indicate that the observed inverse correlation between colon cancer and a moderate red wine intake may be partly mediated by the actions of red wine flavonols on the growth of cancer cells. (C) 2011 Elsevier Ltd. All rights reserved.