Establishment of gastric Helicobacter pylori infection in Mongolian gerbils.
Establishment of gastric Helicobacter pylori infection in Mongolian gerbils.
复制标题
DOI：
--
复制
发表时间：
1996-11
影响因子：
6.3
通讯作者：
F. Hirayama;S. Takagi;Y. Yokoyama;E. Iwao;Y. Ikeda
中科院分区：
文献类型：
--
作者：
F. Hirayama;S. Takagi;Y. Yokoyama;E. Iwao;Y. Ikeda
建立了蒙古沙鼠幽门螺杆菌感染动物模型。对沙鼠口服幽门螺杆菌ATCC 43504培养液。分别于口服接种后第1、7、14、21、42天处死，切除胃。在长达42天的研究期间，所有动物的胃中都保持着幽门螺杆菌的定植。经免疫染色、苏木精染色和伊红染色镜检，胃上皮黏液层可见微生物。所有沙鼠都表现出缓慢进展但典型的胃炎，表现为浅表糜烂，浸润中性粒细胞和单核细胞数量增加。接种后42 d，胃粘膜下层可见淋巴滤泡。这些特征与人类幽门螺杆菌感染的表现相似，因此，该模型对研究幽门螺杆菌致病性和研究人类幽门螺杆菌感染的治疗药物具有一定的价值。
An animal model of Helicobacter pylori infection was obtained with Mongolian gerbils. Culture broth of H. pylori ATCC 43504 was orally administered to the gerbils. Animals were killed 1, 7, 14, 21, and 42 days after the oral inoculation and the stomachs were excised. Colonization of H. pylori was maintained in the stomachs of all animals throughout the study period of up to 42 days. On microscopic examination after immunological and hematoxylin and eosin staining, the organisms were detected in the mucus layer of the gastric epithelium. All gerbils exhibited a slowly progressive but typical gastritis, showing superficial erosion with increasing numbers of infiltrating neutrophils and mononuclear cells. Forty-two days after the inoculation, lymph follicles were detected in the gastric submucosa. These features are similar to the manifestations of H. pylori infection in humans and, accordingly, this model could be of value for studying H. pylori pathogenicity and for research into agents for the treatment of H. pylori infection in humans.