Butanol production by Clostridium beijerinckii.: Part I:: Use of acid and enzyme hydrolyzed corn fiber
Butanol production by Clostridium beijerinckii.: Part I:: Use of acid and enzyme hydrolyzed corn fiber
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DOI：
10.1016/j.biortech.2007.09.087
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发表时间：
2008-09-01
影响因子：
11.4
通讯作者：
Blaschek, Hans P.
中科院分区：
文献类型：
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作者：
Qureshi, Nasib;Ezeji, Thaddeus C.;Blaschek, Hans P.
硫酸处理的玉米纤维水解物（SACFH）的发酵抑制拜氏梭菌（Clostridium beijerinckii）BA 101的细胞生长和丁醇生产（1.7 +/-0.2 g/L丙酮丁醇乙醇或ABE）。用XAD-4树脂处理SACFH去除了一些抑制剂，导致产生9.3 +/-0.5g/L ABE，产率为0.39 +/- 0.015。酶处理的玉米纤维水解物（ETCFH）的发酵没有显示任何细胞抑制，并且导致8.6 +/-1.0g/L ABE的产生和使用24.6g/L总糖。25 g/L葡萄糖和25 g/L木糖发酵的ABE产量分别为9.9 +/- 0.4和9.6 +/- 0.4 g/L，表明培养物能够像葡萄糖一样有效地利用木糖。从XAD-4处理的SACFH仅产生9.3 ± 0.5 g/L ABE（与来自55 g/L葡萄糖对照的发酵的17.7 g/L ABE相比），这表明在处理后可能仍然存在一些发酵抑制剂。建议在用C. beijerinckii BA101.在我们的发酵中，获得了0.35 - 0.39的ABE产率，这高于其他研究者报道的产率。(C)2007爱思唯尔有限公司保留所有权利。
Fermentation of sulfuric acid treated corn fiber hydrolysate (SACFH) inhibited cell growth and butanol production (1.7 +/- 0.2 g/L acetone butanol ethanol or ABE) by Clostridium beijerinckii BA101. Treatment of SACFH with XAD-4 resin removed some of the inhibitors resulting in the production of 9.3 +/- 0.5 g/L ABE and a yield of 0.39 +/- 0.015. Fermentation of enzyme treated corn fiber hydrolysate (ETCFH) did not reveal any cell inhibition and resulted in the production of 8.6 +/- 1.0 g/L ABE and used 24.6 g/L total sugars. ABE production from fermentation of 25 g/L glucose and 25 g/L xylose was 9.9 +/- 0.4 and 9.6 +/- 0.4 g/L, respectively, suggesting that the culture was able to utilize xylose as efficiently as glucose. Production of only 9.3 0.5 g/L ABE (compared with 17.7 g/L ABE from fermentation of 55 g/L glucose-control) from the XAD-4 treated SACFH suggested that some fermentation inhibitors may still be present following treatment. It is suggested that inhibitory components be completely removed from the SACFH prior to fermentation with C. beijerinckii BA101. In our fermentations, an ABE yield ranging from 0.35 to 0.39 was obtained, which is higher than reported by the other investigators. (C) 2007 Elsevier Ltd. All rights reserved.