Highly-sensitive detection of eight typical fluoroquinolone antibiotics by capillary electrophoresis-mass spectroscopy coupled with immunoaffinity extraction
Highly-sensitive detection of eight typical fluoroquinolone antibiotics by capillary electrophoresis-mass spectroscopy coupled with immunoaffinity extraction
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DOI：
10.1039/c7ra12557g
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发表时间：
2018-01
期刊：
影响因子：
3.9
通讯作者：
Xiao-hui Zhang;Yan Deng;Ming-Zhe Zhao;Yinglin Zhou;Xinxiang Zhang
中科院分区：
文献类型：
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作者：
Xiao-hui Zhang;Yan Deng;Ming-Zhe Zhao;Yinglin Zhou;Xinxiang Zhang
氟喹诺酮类（FQs）是一类对支原体和细菌（革兰氏阳性和阴性）具有广谱活性的合成抗生素。由于在兽医和制药行业中广泛使用，环境水样被FQs污染一直受到关注。需要可靠和简单的分析方法来监测这些荧光定量。建立了一种免疫亲和萃取（IAE）-毛细管电泳-质谱（CE-MS）离线同时测定环境水样中8种典型氟喹诺酮类化合物的方法。制备了一种特异性较强的多克隆抗体，并将其固定在Sepharose 4 B柱上进行样品亲和萃取，柱的富集倍数约为100。随后将CE-MS应用于荧光定量物质的分离和定量。在选择性、结合能力、洗脱方案和回收率等参数中评价免疫亲和柱。在优化的条件下，所开发的方法成功地应用于分析荧光定量加标2.5 nM至30 nM的环境水从当地湖泊，提供了令人满意的回收率从72%至112%，和LOD和LOQ范围从1.2 nM至5.0 nM和4.0 nM至17 nM，分别。
Fluoroquinolones (FQs) are a class of synthetic antibiotics with a broad active spectrum towards mycoplasma and bacteria (both Gram positive and negative). The contamination of environmental water samples with FQs has been a concern due to the extensive use of them in the veterinary and pharmaceutical industries. Reliable and simple analytical methods are needed for the monitoring of these FQs. An off-line procedure of immunoaffinity extraction (IAE) followed by capillary electrophoresis-mass spectroscopy (CE-MS) has been developed for simultaneous determination of eight typical FQs in environment water. A broad-specific polyclonal antibody was home-prepared and immobilized on a Sepharose 4B stationary phase for sample affinity extraction and the enrichment factor of our columns was about 100. CE-MS was subsequently applied to FQs separation and quantification. The immunoaffinity column was evaluated in the parameters of selectivity, binding capacity, elution protocol, and recovery. Under the optimized conditions, the developed method was successfully applied to analyze FQs spiked with 2.5 nM to 30 nM of environmental water from a local lake, providing satisfactory recoveries from 72% to 112%, and LODs and LOQs ranging from 1.2 nM to 5.0 nM and 4.0 nM to 17 nM, respectively.