Developmental expression of LPXRFa, kisspeptin, and their receptor mRNAs in the half-smooth tongue sole Cynoglossus semilaevis
Developmental expression of LPXRFa, kisspeptin, and their receptor mRNAs in the half-smooth tongue sole Cynoglossus semilaevis
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DOI：
10.1007/s12562-019-01293-4
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发表时间：
2019-05
影响因子：
1.9
通讯作者：
Bin Wang;Yaxing Zhang;Yongjiang Xu;Xuezhou Liu;Aijun Cui;Bao Shi;Yan Jiang
中科院分区：
文献类型：
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作者：
Bin Wang;Yaxing Zhang;Yongjiang Xu;Xuezhou Liu;Aijun Cui;Bao Shi;Yan Jiang
促性腺激素抑制激素(GnIH)和Kispeptin在脊椎动物的生殖控制中都起着关键作用。然而，这两个肽在发育过程中的时空表达模式和可能的作用还知之甚少。为此，我们研究了半滑舌鱼(Cynoglossus Semilaevis)胚胎发生和早期幼体发育过程中，pxrfa、GniH和kisspeptin(Kiss2)及其受体mRNAs的动态表达谱。定量RT-PCR分析表明，在未受精卵和胚胎发育过程中都检测到了配体(Lpxrfaandkiss2)和受体(lpxrfa-rankiss2r)mRNAs，但表达谱不同。此外，在孵化后6天(DPH)，lpxrfa-r、kiss2和kiss2r转录本显著增加，而elpxrFamRNAs在所有幼虫发育过程中变化不大。综上所述，本研究结果表明，LPXRFa和Kispeptin系统在舌底的胚胎发生和早期幼体发育过程中可能具有不同的功能，并发挥不同的作用。
Both gonadotropin-inhibitory hormone (GnIH) and kisspeptin play a critical role in the control of reproduction in vertebrates. However, the spatial–temporal expression patterns and possible roles of these two peptides during development are poorly understood. To this end, we evaluated dynamic expression profiles oflpxrfa, the piscine ortholog ofgnih, kisspeptin (kiss2) and their receptor mRNAs during embryogenesis and early larval development in a flatfish,Cynoglossus semilaevis. Quantitative RT-PCR analysis indicated that both ligand (lpxrfaandkiss2) and receptor (lpxrfa-randkiss2r) mRNAs were detected in unfertilized eggs and during embryogenesis, but with different expression profiles. In addition,lpxrfa-r,kiss2andkiss2rtranscripts increased significantly at 6 days post-hatching (dph), whilelpxrfamRNAs did not vary significantly during all larval development. Taken together, these results of the present study suggest that both LPXRFa and kisspeptin systems could be functional and play differential roles during embryogenesis and early larval development in tongue sole.