Facial nerve palsy after reactivation of herpes simplex virus type 1 in diabetic mice
Facial nerve palsy after reactivation of herpes simplex virus type 1 in diabetic mice
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糖尿病小鼠 1 型单纯疱疹病毒重新激活后面神经麻痹
DOI：
10.1002/lary.24994
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Murakami S
中科院分区：
文献类型：
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作者：
Esaki S;Yamano K;Katsumi S;Minakata T;Murakami S
目的/假设贝尔麻痹与糖尿病(DM)密切相关。单纯疱疹病毒1型(HSV-1)的重新激活或糖尿病单神经病变被认为是导致DM患者面瘫的原因。然而，很难区分面瘫是由疱疹病毒性神经炎还是糖尿病单神经病变引起的。我们之前曾报道，在接种HSV-1小鼠耳廓后，DM小鼠面神经麻痹加重。方法用链脲佐菌素(STZ)诱导糖尿病小鼠面神经一过性瘫痪后注射链脲佐菌素诱导糖尿病，耳部抓抓HSV-1引起复发性面瘫。结果DM诱导后，糖尿病小鼠CD3+T细胞数量减少70%，13%的DM小鼠面神经麻痹复发。单纯疱疹病毒1型脱氧核糖核酸(DNA)在所有DM面瘫小鼠面神经中均可检测到，在膝状神经节的电子显微镜下可见HSV-1衣壳。结论单纯疱疹病毒1型在膝状神经节重新激活可能是糖尿病面神经麻痹发生率增加的主要原因。
Objectives/HypothesisBell's palsy is highly associated with diabetes mellitus (DM). Either the reactivation of herpes simplex virus type 1 (HSV‐1) or diabetic mononeuropathy has been proposed to cause the facial paralysis observed in DM patients. However, distinguishing whether the facial palsy is caused by herpetic neuritis or diabetic mononeuropathy is difficult. We previously reported that facial paralysis was aggravated in DM mice after HSV‐1 inoculation of the murine auricle. In the current study, we induced HSV‐1 reactivation by an auricular scratch following DM induction with streptozotocin (STZ).Study DesignControlled animal study.MethodsDiabetes mellitus was induced with streptozotocin injection in only mice that developed transient facial nerve paralysis with HSV‐1. Recurrent facial palsy was induced after HSV‐1 reactivation by auricular scratch.ResultsAfter DM induction, the number of cluster of differentiation 3 (CD3)+T cells decreased by 70% in the DM mice, and facial nerve palsy recurred in 13% of the DM mice. Herpes simplex virus type 1 deoxyribonucleic acid (DNA) was detected in the facial nerve of all of the DM mice with palsy, and HSV‐1 capsids were found in the geniculate ganglion using electron microscopy. Herpes simplex virus type 1 DNA was also found in some of the DM mice without palsy, which suggested the subclinical reactivation of HSV‐1.ConclusionsThese results suggested that HSV‐1 reactivation in the geniculate ganglion may be the main causative factor of the increased incidence of facial paralysis in DM patients.Level of EvidenceN/A.Laryngoscope, 125:E143–E148, 2015