Quantitative analysis of biopolymers by matrix-assisted laser desorption
Quantitative analysis of biopolymers by matrix-assisted laser desorption
复制标题
通过基质辅助激光解吸对生物聚合物进行定量分析
DOI：
10.1021/ac00063a041
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发表时间：
1993
影响因子：
7.4
通讯作者：
Chung
中科院分区：
文献类型：
--
作者：
K. Tang;S. Allman;R. Jones;Chung
在过去的几年中，由于对生物和医学研究的巨大潜在益处，已经做出了重大努力来使用质谱法测量生物聚合物。虽然MALDI的激光解吸和电离机制的理论细节尚未完全理解，但已经提出了几种模型来解释大的生物聚合物离子的产生。简而言之，很难通过MALDI获得分析物绝对量的可靠测量。如果MALDI要成为一种常规的分析工具，显然必须追求定量测量能力。本工作中使用的寡核苷酸和蛋白质样品购自商业来源。烟酸被用作这两种类型的生物聚合物的基质。从该实验可以看出，使用MALDI难以获得生物聚合物的绝对定量测量。然而，使用具有相似化学性质的分子的内部校准可用于解决这些困难。必须避免生物聚合物之间的化学反应，以防止分析物材料的破坏。参考文献10篇，图8。
During the past few years, major efforts have been made to use mass spectrometry to measure biopolymers because of the great potential benefit to biological and medical research. Although the theoretical details of laser desorption and ionization mechanisms of MALDI are not yet fully understood, several models have been presented to explain the production of large biopolymer ions. In brief, it is very difficult to obtain reliable measurements of the absolute quantity of analytes by MALDI. If MALDI is going to become a routine analytical tool, it is obvious that quantitative measurement capability must be pursued. Oligonucleotides and protein samples used in this work were purchased from commercial sources. Nicotinic acid was used as matrix for both types of biopolymers. From this experiment, it is seen that it is difficult to obtain absolute quantitative measurements of biopolymers using MALDI. However, internal calibration with molecules having similar chemical properties can be used to resolve these difficulties. Chemical reactions between biopolymers must be avoided to prevent the destruction of the analyte materials. 10 refs., 8 figs.