Movement of cortical actin patches in yeast.
Movement of cortical actin patches in yeast.
复制标题
酵母中皮质肌动蛋白斑块的运动。
DOI：
10.1083/jcb.132.5.861
复制
发表时间：
1996-03
影响因子：
7.8
通讯作者：
Cooper, J A
中科院分区：
文献类型：
--
作者：
Waddle, J A;Karpova, T S;Waterston, R H;Cooper, J A
在酵母中，肌动蛋白形成与质膜相关的斑块。斑块分布与细胞周期中的极化生长和对外界刺激的反应相关。使用绿色荧光蛋白融合到加帽蛋白成像活细胞中的肌动蛋白斑块，我们发现斑块移动迅速，距离较长。即使是成簇的斑块，如在萌芽部位，也显示出运动。斑块彼此独立移动，通常在局部区域内移动很小的距离，但它们也可以移动更大的距离，包括通过母芽颈。在细胞周期中，斑块极化的变化迅速发生。这些观察结果提供了重要的新参数的肌动蛋白的调节和功能的分子分析。
In yeast, actin forms patches associated with the plasma membrane. Patch distribution correlates with polarized growth during the cell cycle and in response to external stimuli. Using green fluorescent protein fused to capping protein to image actin patches in living cells, we find that patches move rapidly and over long distances. Even patches in clusters, such as at the incipient bud site, show movement. Patches move independently of one another and generally over small distances in a local area, but they can also move larger distances, including through the mother-bud neck. Changes in patch polarization occur quickly through the cell cycle. These observations provide important new parameters for a molecular analysis of the regulation and function of actin.