Dynamic expression pattern of Hoxc8 during mouse early embryogenesis
Dynamic expression pattern of Hoxc8 during mouse early embryogenesis
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DOI：
10.1002/ar.a.20160
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发表时间：
2005-03-01
期刊：
影响因子：
--
通讯作者：
Kim, AH
中科院分区：
文献类型：
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作者：
Kwon, YJ;Shin, J;Kim, AH
应用原位杂交和RT-PCR技术检测了Hoxc 8在小鼠胚胎移植后7.5-12.5天（dpc）的表达情况。Hoxc 8的表达在7.5和8.5 dpc之间开始。在8.5 dpc时，在外胚层和中胚层中检测到强表达。在9.5 dpc，一个明显的前边界Hoxc 8表达的神经管和近轴中胚层，分别在第10和第16体节。这种交错的表达模式在整个后期阶段都保持着。到12.5 dpc时，观察到Hoxc 8表达模式向前进展，染色减弱。在外胚层来源的神经管，强烈的Hoxc 8表达观察到在腹角，后来在腹侧和内侧区域的外套层，表明可能与神经分化的发生和进展。在中胚层衍生细胞的情况下，在通往脊索、神经管和中肾的途中的硬结中检测到强烈的Hoxc 8表达，这表明Hoxc 8在椎骨和肋骨的形成中的作用以及可能参与分化成肾。(C)2005 Wiley-Liss，Inc.
The Hoxc8 expression pattern was examined in mouse embryos 7.5-12.5 days postcoitum (dpc) using whole-mount in situ hybridization and RT-PCR. The expression of Hoxc8 started between 7.5 and 8.5 dpc. A strong expression was detected in the ectoderm and mesoderm at 8.5 dpc. At 9.5 dpc, a distinct anterior boundary of Hoxc8 expression was established at the 10th and 16th somites in the neural tube and the paraxial mesoderm, respectively. This staggered expression pattern was maintained throughout the later stages. By 12.5 dpc, the for-ward progression of the Hoxc8 expression pattern was observed and the stain was weakened. In the ectoderm-derived neural tube, strong Hoxc8 expression was observed in the ventral horn and later in the ventral and mediolateral region of the mantle layer, indicating a possible association with the onset and progression of neural differentiation. In the case of the mesoderm-derivative cells, strong Hoxc8 expression was detected in the sclerotome on the way to the notochord and neural tube and mesonephros, suggesting a role of Hoxc8 in the formation of the vertebrae and ribs and the possible involvement in the differentiation into the kidney. (C) 2005 Wiley-Liss, Inc.