Metformin improves the glucose homeostasis of Wuchang bream fed high-carbohydrate diets: a dynamic study
Metformin improves the glucose homeostasis of Wuchang bream fed high-carbohydrate diets: a dynamic study
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DOI：
10.1530/ec-18-0517
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发表时间：
2019-01
影响因子：
2.9
通讯作者：
Chao Xu;Xiang‐fei Li;Hongyan Tian;Hua‐Juan Shi;Dingdong Zhang;K. Abasubong;Wen‐bin Liu
中科院分区：
文献类型：
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作者：
Chao Xu;Xiang‐fei Li;Hongyan Tian;Hua‐Juan Shi;Dingdong Zhang;K. Abasubong;Wen‐bin Liu
在为期12周的饲养试验后，对团头鲂进行糖耐量试验，以评价二甲双胍对糖脂代谢反应的影响。血浆胰岛素在注射后2 h达到峰值，然后在注射后8-12 h降至基础值。对照组的血浆甘油三酯水平和肝糖原含量分别在前2和1 h显著降低。然后，在12 h时恢复至基础值。在整个采样期间，高碳水化合物组的血浆代谢物和肝糖原水平显著高于对照组，二甲双胍补充剂增强了这些变化（胰岛素水平除外）。葡萄糖可使AMPK α1、AMPK α2、pepck、g6 pase、fbpase、cptIA和aco的转录、AMPK α的磷酸化和促凋亡酶活性在2-4 h内降低，而GLUT 2、gs、gk、pk、accα和fas则相反。高碳水化合物日粮在整个取样期内显著提高了ampk α1、ampk α2、glut 2、gs、gk、pk、accα和fas的转化率，Ampk α的磷酸化水平和糖酵解酶的活性，而其余指标则相反。此外，二甲双胍显著上调上述指标（accα和fas除外）以及cpt IA和aco的转运。总体而言，二甲双胍通过激活Ampk和刺激糖酵解、糖原生成和脂肪酸氧化，同时抑制脂肪生成和脂肪生成，有利于高糖饲料喂养团头鲂的葡萄糖稳态。
After a 12-week feeding trial, the glucose tolerance test was performed in Megalobrama amblycephala to evaluate the effects of metformin on the metabolic responses of glycolipids. Plasma insulin peaked at 2 h, then decreased to the basal value at 8–12 h post-injection. Plasma triglyceride levels and liver glycogen contents of the control group was decreased significantly during the first 2 and 1 h, respectively. Then, they returned to basal values at 12 h. During the whole sampling period, the high-carbohydrate groups had significantly higher levels of plasma metabolites and liver glycogen than those of the control group, and metformin supplementation enhanced these changes (except insulin levels). Glucose administration lowered the transcriptions of ampk α1, ampk α2, pepck, g6pase, fbpase, cpt IA and aco, the phosphorylation of Ampk α and the activities of the gluconeogenic enzymes during the first 2–4 h, while the opposite was true of glut 2, gs, gk, pk, accα and fas. High-carbohydrate diets significantly increased the transcriptions of ampk α1, ampk α2, glut 2, gs, gk, pk, accα and fas, the phosphorylation of Ampk α and the activities of the glycolytic enzymes during the whole sampling period, while the opposite was true for the remaining indicators. Furthermore, metformin significantly upregulated the aforementioned indicators (except accα and fas) and the transcriptions of cpt IA and aco. Overall, metformin benefits the glucose homeostasis of Megalobrama amblycephala fed high-carbohydrate diets through the activation of Ampk and the stimulation of glycolysis, glycogenesis and fatty acid oxidation, while depressing gluconeogenesis and lipogenesis.