Effect of cell media on polymer coated superparamagnetic iron oxide nanoparticles (SPIONs): Colloidal stability, cytotoxicity, and cellular uptake studies
Effect of cell media on polymer coated superparamagnetic iron oxide nanoparticles (SPIONs): Colloidal stability, cytotoxicity, and cellular uptake studies
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DOI：
10.1016/j.ejpb.2007.02.024
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发表时间：
2008-01-01
影响因子：
4.9
通讯作者：
Hofmann, Heinrich
中科院分区：
文献类型：
--
作者：
Petri-Fink, Alke;Steitz, Benedikt;Hofmann, Heinrich
本文报道了聚合物包覆聚乙烯醇（PVA）、乙烯醇/乙烯胺共聚物（A-PVA）和聚乙烯亚胺（PEI）的超顺磁性氧化铁纳米粒子（SPION）的组成对这些粒子在不同细胞介质中的胶体稳定性、细胞毒性和细胞摄取的影响。尽管所有检测的聚合物包被SPION在水和PBS缓冲液中稳定，但这些胶体系统在不含和补充有胎牛血清（FCS）的DMEM或RPMI培养基中具有不同的稳定性。我们发现，与单独使用相同浓度的A-PVA相比，SPION表面上的A-PVA涂层降低了聚合物的细胞毒性。同样，与PEI和PEI-SPION相比，具有用于转染实验的浓度的DNA的PEI-SPION的聚合复合物没有显示出细胞毒性。我们的数据表明，介质的选择在很大程度上影响这些颗粒的HeLa细胞的摄取。对于不同的受检聚合物涂层SPION，最佳培养基是不同的，并且应在每种情况下单独确定。(c)2007 Elsevier B. V.保留所有权利。
The influence of the composition of the polymer coated polyvinyl alcohol (PVA), vinyl alcohol/vinyl amine copolymer (A-PVA) and polyethylenimine (PEI) coated superparamagnetic iron oxide nanoparticles (SPIONs) on the colloidal stability, cytotoxicity and cellular uptake of these particles in different cell media is reported in this paper. Although all examined polymer coated SPIONs were stable in water and PBS buffer these colloidal systems had different stabilities in DMEM or RPMI media without and supplemented with fetal calf serum (FCS). We found that A-PVA coating onto the surface of the SPIONs decreased the cytotoxicity of the polymer compared to the same concentration of A-PVA alone. As well, polyplexes of PEI-SPIONs with DNA in concentration used for transfection experiments showed no cytotoxicity compared to PEI and PEI-SPIONs. Our data show that the choice of medium largely influences the uptake of these particles by HeLa cells. The optimal medium is different for the different examined polymer coated SPIONs and it should be determined in each case, individually. (c) 2007 Elsevier B.V. All rights reserved.