Chemiluminescence high-performance liquid chromatographic determination of tea catechin, (-)-epigallocatechin 3-gallate, at picomole levels in rat and human plasma
Chemiluminescence high-performance liquid chromatographic determination of tea catechin, (-)-epigallocatechin 3-gallate, at picomole levels in rat and human plasma
复制标题
DOI：
10.1006/abio.1997.2098
复制
发表时间：
1997-05-15
影响因子：
2.9
通讯作者：
Miyazawa, T
中科院分区：
文献类型：
--
作者：
Nakagawa, K;Miyazawa, T
建立了一种高效液相色谱-化学发光检测法（CL-HPLC）测定大鼠和人体血浆中儿茶素-表没食子儿茶素没食子酸酯（EGCg）的新方法。8.2M乙醛溶于50 mM磷酸盐缓冲液（pH7.4，含辣根过氧化物酶108 mg/L）和8.8M过氧化氢水溶液中，用甲醇提取血浆EGCg。该方法能够选择性地检测游离形式的EGCg，回收率为84%，低至2 pmol。空腹大鼠血浆中EGCg浓度最初低于检测限（
A method of chemiluminescence detection-highperformance liquid chromatography (CL-HPLC) for the highly specific determination of tea catechin, (-)epigallocatechin 3-gallate (EGCg), present in rat and human plasma has been newly developed, The CL-HPLC system consists of reversed-phase HPLC and chemiluminescence detector, in which separated EGCg generates chemiluminescence at post column successively reacting with the following two chemiluminescence cocktails; 8.2 M acetaldehyde in 50 mM phosphate buffer (pH 7.4, contained 108 mg horseradish peroxidase/L) and 8.8 M hydrogen peroxide aqueous solution, The plasma EGCg was extracted by methanol. This method enables the detection of EGCg in the free form selectively at as low as 2 pmol with recovery of 84%. The EGCg concentration in fasted rat plasma was initially below the detection limit (