Evolutionary Screening of Collagen-like Peptides That Nucleate Hydroxyapatite Crystals
Evolutionary Screening of Collagen-like Peptides That Nucleate Hydroxyapatite Crystals
复制标题
DOI：
10.1021/la104757g
复制
发表时间：
2011-06-21
期刊：
影响因子：
3.9
通讯作者：
Lee, Seung-Wuk
中科院分区：
文献类型：
--
作者：
Chung, Woo-Jae;Kwon, Ki-Young;Lee, Seung-Wuk
无机/有机复合材料（如骨）的生物发生通常涉及模板矿化过程。因此，仿生合成骨样材料需要开发介导骨的主要无机成分羟基磷灰石（HAP）矿化的有机支架。使用噬菌体展示，我们确定了一个12个残基的肽，结合到单晶HAP和模板的结晶HAP矿物的成核和生长的序列和组成依赖性的方式。负责矿化活性的序列类似于I型胶原的三肽重复（Gly-Pro-Hyp），I型胶原是骨细胞外基质的主要成分。使用一组合成肽，我们定义了矿化活性所需的结构特征。结果支持了一个模型的合作非共价相互作用的肽与HAP，并建议，天然胶原蛋白可能有一个矿物质模板功能在体内。我们期望这种短的HAP结合肽在三维骨样材料的合成中是有用的。
The biogenesis of inorganic/organic composite materials such as bone typically involves the process of templated mineralization. Biomimetic synthesis of bone-like materials therefore requires the development of organic scaffolds that mediate mineralization of hydroxyapatite (HAP), the major inorganic component of bone. Using phage display, we identified a 12-residue peptide that bound to single-crystal HAP and templated the nucleation and growth of crystalline HAP mineral in a sequence- and composition-dependent manner. The sequence responsible for the mineralizing activity resembled the tripeptide repeat (Gly-Pro-Hyp) of type I collagen, a major component of bone extracellular matrix. Using a panel of synthetic peptides, we defined the structural features required for mineralizing activity. The results support a model for the cooperative noncovalent interaction of the peptide with HAP and suggest that native collagen may have a mineral-templating function in vivo. We expect this short HAP-binding peptide to be useful in the synthesis of three-dimensional bone-like materials.