A gene near the plasmid pSa origin of replication encodes a nuclease.
A gene near the plasmid pSa origin of replication encodes a nuclease.
复制标题
质粒 pSa 复制起点附近的基因编码核酸酶。
DOI：
10.1111/j.1365-2958.1992.tb01497.x
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发表时间：
1992
影响因子：
3.6
通讯作者：
Kado,CI
中科院分区：
文献类型：
--
作者：
Close,SM;Kado,CI
我们已经从IncW质粒pSa中克隆并测序了一个基因（nuc），该基因显示出与葡萄球菌核酸酶（EC 3.1.4.7）和质粒RP 4的parBlocus的氨基酸序列相似性。该基因的开放阅读框为525 bp，编码19.7 kDa的174个氨基酸。使用产生相同大小的蛋白质的体外转录-翻译测定证实基因的表达。在甲苯胺蓝-DNA琼脂平板中使用DNA作为底物证明核酸酶活性。推导的氨基酸序列揭示了一个信号序列，并且TnphoA插入在开放阅读框内表明蛋白质的一部分被转运穿过细菌细胞膜。
We have cloned and sequenced a gene(nuc)from the IncW plasmid pSa which shows amino acid sequence similarity to staphylococcal nuclease (EC 3.1.4.7) and to theparBlocus of plasmid RP4. The 525bp open reading frame encodes a 174‐amino‐acid potential polypeptide of 19.7kDa. Expression of the gene was confirmed using anin vitrotranscription‐translation assay which produced a protein of identical size. Nuclease activity was demonstrated using DNA as the substrate in toluidine blue‐DNA agar plates. The deduced amino acid sequence revealed a signal sequence, andTnphoAinsertion within the open reading frame indicated that a portion of the protein is transported across the bacterial cell membrane.