C-terminal phosphorylation of the high molecular weight neurofilament subunit correlates with decreased neurofilament axonal transport velocity
C-terminal phosphorylation of the high molecular weight neurofilament subunit correlates with decreased neurofilament axonal transport velocity
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DOI：
10.1016/s0006-8993(99)02314-8
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发表时间：
2000-02-21
期刊：
影响因子：
2.9
通讯作者：
Shea, TB
中科院分区：
文献类型：
--
作者：
Jung, C;Yabe, JT;Shea, TB
我们通过比较2个月龄和5个月龄的两组年轻成年小鼠的这些参数，探讨了神经丝的神经纤维轴突运输与它们的磷酸化状态之间的关系。之所以选择这个特定的时间间隔，是因为先前的研究表明，视神经轴突已经完成了轴突口径的扩张，并在2个月时达到成年的神经营养因子水平，但如这里所示，继续增加神经营养因子-H-C末端的磷酸化。玻璃体内注射S-35-蛋氨酸后，通过放射自显影分析从视神经轴突节段每隔一段时间免疫沉淀的放射性标记亚单位的分布，监测神经纤维的轴突运输。与5月龄的小鼠相比，2个月龄的小鼠核素标记的神经纤维峰和前额移位更快。神经纤维转运率的这种发育下降并不是由于细胞骨架结合神经营养因子的减少，也不是由于缓慢的轴突运输的整体下降。通过排除或最小化其他因素，这些发现支持了先前的结论，即C末端的NF磷酸化调节了NF的轴突运输。(C)2000由爱思唯尔科学公司出版。保留所有权利。
We probed the relationship of NF axonal transport of neurofilaments (NFs) to their phosphorylation state by comparing these parameters in two closely-aged groups of young adult mice - 2 and 5 months of age. This particular time interval was selected since prior studies demonstrate that optic axons have already completed axonal caliber expansion and attained adult NF levels by 2 months but, as shown herein, continue to increase NF-H C-terminal phosphorylation. NF axonal transport was monitored by autoradiographic analysis of the distribution of radiolabeled subunits immunoprecipitated from optic axon segments at intervals following intravitreal injection of S-35-methionine. Both the peak and front of radiolabeled NFs translocated Faster in 2- vs. 5-month-old mice. This developmental decline in NF transport rate was not due to reduced incorporation of NFs into the cytoskeleton, nor to an overall decline in slow axonal transport. By excluding or minimizing other factors, these findings support previous conclusions that C-terminal NF phosphorylation regulates NF axonal transport. (C) 2000 Published by Elsevier Science B.V. All rights reserved.