Natural killer cell function and malignant cell phenotype in hairy cell leukaemia
Natural killer cell function and malignant cell phenotype in hairy cell leukaemia
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毛细胞白血病的自然杀伤细胞功能和恶性细胞表型
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
Ó. Jensson
中科院分区：
文献类型：
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作者：
H. Ögmundsdóttir;L. Thorsteinsson;A. Sigfússon;S. Sveinsdóttir;S. Björnsson;G. Eyjolfsson;G. Jóhannesson;Ó. Jensson
我们使用单细胞测定和微细胞毒性测定，对毛细胞白血病 (HCL) 患者 (A) 的自然杀伤 (NK) 细胞数量和活性发生的变化进行了 33 个月的跟踪。还分析了外周血单核细胞群的组成和恶性细胞表型。在此期间，他接受了干扰素治疗，并切除了严重肿大的脾脏。还对另外四名患者进行了研究，其中两名患者被切除了脾脏，并且所有人都接受了干扰素治疗。在所研究的 5 名患者中，有 4 名患者的 NK 活性低与肿瘤浸润的脾脏之间存在明显的联系，而在第五名患有白血病且无脾肿大的患者中，NK 功能与疾病活动相关。这五名患者的 NK 活性与靶结合 (TB) 细胞数量之间没有相关性。 IFN对整体NK活性几乎没有直接影响，但TB细胞中杀伤细胞的比例有所增加。三名患者表现出多个细胞与单个靶标的结合。进一步分析显示，与正常受试者的结核细胞相比，患者中的大多数结核细胞不是 CD56 阳性 NK 细胞。在患者 A 中，很大一部分 (84%) 的 TB 细胞被鉴定为恶性细胞，在患者 E 中，15% 的 TB 细胞被鉴定为恶性细胞。除患者A外，恶性细胞的表型为：CD19+、HLA-DR+和CD25(Tac)+。该患者的毛细胞NK标记物CD56和单核细胞标记物CD14呈阳性。此外，表型发生了变化，因为只有后来的样本才携带 CD25。结论是，NK 功能水平与 HCL 中的疾病活动密切相关，恶性细胞的竞争性靶细胞结合可能是毛细胞白血病中 NK 细胞功能低下的原因之一。
We have followed for 33 months the changes that occurred in natural killer (NK) cell numbers and activity in a patient (A) with hairy cell leukaemia (HCL), using a single cell assay and a microcytotoxicity assay. The composition of the peripheral blood mononuclear cell population and malignant cell phenotype were also analysed. During this period he received treatment with interferon and his grossly enlarged spleen was removed. Four further patients were also studied, two were splenectomized and all had received treatment with interferon. In four of the five patients studied there was an apparent link between low NK activity and presence of a tumour‐infiltrated spleen, and in the fifth patient, who was aleukemic and had no splenomegaly, NK function was related to disease activity. There was no correlation between NK activity and the number of target binding (TB) cells in these five patients. IFN had little direct effect on overall NK activity, but the proportion of killing cells among TB cells was increased. Three patients showed binding of several cells to a single target. Further analysis revealed that in the patients most of the TB cells were not CD56‐positive NK cells, in contrast to TB cells from normal subjects. In patient A a large proportion (84%) of TB cells were identified as malignant cells and in patient E 15% of TB cells were malignant cells. The phenotype of the malignant cells was: CD19 +, HLA‐DR + and CD25(Tac) +, except for patient A. In this patient the hairy cells were positive for the NK marker CD56 as well as the monocyte marker CD14. Furthermore, a change occurred in phenotype as only later samples carried CD25. It is concluded that the level of NK function correlates closely with disease activity in HCL and that competitive target cell binding by malignant cells may be one cause of depressed NK‐cell function in hairy cell leukaemia.
毛细胞白血病患者的肿瘤性 B 细胞产生 B 细胞生长因子。
影响因子：
20.3
作者：
Ford,RJ;Kwok,D;Quesada,J;Sahasrabuddhe,CG
通讯作者：
Sahasrabuddhe,CG