Microstructural imaging in the spinal cord and validation strategies
Microstructural imaging in the spinal cord and validation strategies
复制标题
DOI：
10.1016/j.neuroimage.2018.04.009
复制
发表时间：
2018-11-15
期刊：
影响因子：
5.7
通讯作者：
Cohen-Adad, J.
中科院分区：
文献类型：
--
作者：
Cohen-Adad, J.
利用磁共振成像（MRI）的体内组织学是一个新兴的研究领域，其目的是非侵入性地表征组织的微观结构。体内组织学的意义很多，从发现新的生物标志物到研究人类发育，再到提供疾病诊断工具和监测新治疗对组织的影响。本文综述了定量MRI（qMRI）技术，用于映射脊髓显微结构。本文首先介绍了脊髓非侵入性成像的基本原理，然后简要概述了用于轴突和髓鞘成像的现有MRI技术，随后介绍了获取和处理此类数据的具体挑战和潜在解决方案。本综述的最后一部分侧重于组织学验证，建议组织制备，采集和处理协议的大规模显微镜。
In vivo histology using magnetic resonance imaging (MRI) is a newly emerging research field that aims to non-invasively characterize tissue microstructure. The implications of in vivo histology are many, from discovering novel biomarkers to studying human development, to providing tools for disease diagnosis and monitoring the effects of novel treatments on tissue. This review focuses on quantitative MRI (qMRI) techniques that are used to map spinal cord microstructure. Opening with a rationale for non-invasive imaging of the spinal cord, this article continues with a brief overview of the existing MRI techniques for axon and myelin imaging, followed by the specific challenges and potential solutions for acquiring and processing such data. The final part of this review focuses on histological validation, with suggested tissue preparation, acquisition and processing protocols for large-scale microscopy.