Medullary respiratory neurones and control of laryngeal motoneurones during fictive eupnoea and cough in the cat
Medullary respiratory neurones and control of laryngeal motoneurones during fictive eupnoea and cough in the cat
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DOI：
10.1111/j.1469-7793.2001.t01-1-00565.x
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发表时间：
2001-07-15
影响因子：
5.5
通讯作者：
Shannon, R
中科院分区：
文献类型：
--
作者：
Baekey, DM;Morris, KF;Shannon, R
1.本研究提出了腹外侧延髓呼吸神经元参与正常呼吸和咳嗽期间喉部运动神经元控制的假设。2．数据来自 28 只中丘去大脑、人工通气的猫。通过胸内气管的机械刺激引起膈神经、腰神经和喉返神经的咳嗽样运动模式（虚构咳嗽）。微电极阵列用于同时监测腹侧呼吸组的多个神经元，包括 Botzinger 和前 Botzinger 复合体。评估了尖峰序列在虚构咳嗽期间的反应以及尖峰触发的传出喉返神经活动平均值的功能连接证据。3．主要特征是在 332 个神经元中的 94 个 (28%) 神经元触发的平均值中观察到的。 10 个吸气神经元和 13 个呼气神经元的未校正平均值中存在具有正时滞的偏置双相波。这些触发神经元分别被识别为具有增强、递减、平台和“其他”放电模式的吸气喉运动神经元和具有递减放电模式的呼气喉运动神经元4。由吸气神经元触发的校正平均值包括 37 个偏移峰、11 个中心峰和 1 个偏移谷。由呼气神经元触发的平均值有 12 个偏移峰、6 个中心峰和 4 个偏移谷。从这些特征推断出的关系包括吸气神经元对吸气喉运动神经元具有增强、递减、平台和“其他”模式的运动前动作，以及递减和“其他”呼气神经元对呼气喉运动神经元的运动前动作。虚构咳嗽期间神经元放电模式的相应变化支持了这些推论。5。这些数据证实并扩展了先前关于平喘期间喉部运动神经元控制的结果，并支持以下假设：相同的前运动神经元有助于塑造平喘和咳嗽期间的运动神经元放电模式。
1. This study addressed the hypothesis that ventrolateral medullary respiratory neurones participate, in the control of laryngeal motoneurones during both eupnoea and coughing.2. Data were obtained from 28 mid-collicular decerebrated, artificially ventilated cats. Cough-like motor patterns (fictive cough) in phrenic, lumbar and recurrent laryngeal nerves were elicited by mechanical stimulation of the intrathoracic trachea. Microelectrode arrays were used to monitor simultaneously several neurones in the ventral respiratory group, including the Botzinger and pre-Botzinger complexes. Spike trains were evaluated for responses during fictive cough and evidence of functional connectivity with spike-triggered averages of efferent recurrent laryngeal nerve activity.3. Primary features were observed in averages triggered by 94 of 332 (28 %) neurones. An offset biphasic wave with a positive, time lag was present in the unrectified average for 10 inspiratory and 13 expiratory neurones. These trigger neurones were respectively identified as inspiratory laryngeal motoneurones with augmenting, decrementing, plateau and 'other' discharge patterns, and expiratory laryngeal motoneurones with decrementing firing patterns,4. Rectified averages triggered by inspiratory neurones included 37 offset peaks, 11 central peaks and one offset trough. Averages triggered by expiratory neurones had 12 offset peaks, six central peaks and four offset troughs. Relationships inferred from these features included premotor actions of inspiratory neurones with augmenting, decrementing, plateau and 'other' patterns on inspiratory laryngeal motoneurones, and premotor actions of decrementing and 'other' expiratory neurones on expiratory laryngeal motoneurones. Corresponding changes in neuronal firing patterns during fictive cough supported these inferences.5. The data confirm and extend previous results on the control of laryngeal motoneurones during eupnoea and support the hypothesis that the same premotor neurones help to shape motoneurone firing patterns during both eupnoea and coughing.