In vitro acylation of okadaic acid in the presence of various bivalves' extracts.
In vitro acylation of okadaic acid in the presence of various bivalves' extracts.
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DOI：
10.3390/md11020300
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发表时间：
2013-01-29
期刊：
影响因子：
5.4
通讯作者：
Yotsu-Yamashita M
中科院分区：
文献类型：
--
作者：
Konoki K;Onoda T;Watanabe R;Cho Y;Kaga S;Suzuki T;Yotsu-Yamashita M
甲藻Dinophysis spp.是导致贝类中毒的罪魁祸首。在暴露于甲藻的有毒水华的双壳类中，产生甲藻毒素3（DTX 3），其为冈田酸（OA）或DTX 1的7-OH酰化形式。我们证明了在体外酰化的OA与棕榈酰CoA的存在下，从消化腺的蛋白质提取物，但不是其他组织的双壳海贝虾夷扇贝。7-O-棕榈酰OA的产率在2 h内达到最大值，在37 °C下最高，随后是28 °C、16 °C和4 °C，并且与在pH 6和pH 4下的产率相比，在pH 8下最高。当从双壳贝类Corbicula japonica和Crassostrea gigas制备蛋白质提取物时，也进行了转化。OABP 2在冈田软海绵中被鉴定，但在双壳类M中未检测到。yessoensis、双壳类贻贝Mytilus galloprovincialis和海鞘Halocynthia roretzi，尽管已知它们积累了有毒的贝类毒素。由于DTX 3不结合蛋白磷酸酶1和2A，在哺乳动物细胞中的OA和DTXs的生理目标，DSP毒素的酰化将与双壳类的解毒机制。
The dinoflagellate Dinophysis spp. is responsible for diarrhetic shellfish poisoning (DSP). In the bivalves exposed to the toxic bloom of the dinoflagellate, dinophysistoxin 3 (DTX3), the 7-OH acylated form of either okadaic acid (OA) or DTX1, is produced. We demonstrated in vitro acylation of OA with palmitoyl CoA in the presence of protein extract from the digestive gland, but not other tissues of the bivalve Mizuhopecten yessoensis. The yield of 7-O-palmitoyl OA reached its maximum within 2 h, was the highest at 37 °C followed by 28 °C, 16 °C and 4 °C and was the highest at pH 8 in comparison with the yields at pH 6 and pH 4. The transformation also proceeded when the protein extract was prepared from the bivalves Corbicula japonica and Crassostrea gigas. The OA binding protein OABP2 identified in the sponge Halichondria okadai was not detected in the bivalve M. yessoensis, the bivalve Mytilus galloprovincialis and the ascidian Halocynthia roretzi, though they are known to accumulate diarrhetic shellfish poisoning toxins. Since DTX3 does not bind to protein phosphatases 1 and 2A, the physiological target for OA and DTXs in mammalian cells, the acylation of DSP toxins would be related to a detoxification mechanism for the bivalve species.
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影响因子：
5.1
作者：
HU, TM;MARR, J;PLEASANCE, S
通讯作者：
PLEASANCE, S
DOI：
10.1039/c39920000039
发表时间：
1992-01-01
影响因子：
--
作者：
HU, TM;DOYLE, J;WRIGHT, JLC
通讯作者：
WRIGHT, JLC
影响因子：
2.9
作者：
Sugiyama, Naoyuki;Konoki, Keiichi;Tachibana, Kazuo
通讯作者：
Tachibana, Kazuo
影响因子：
2.8
作者：
Suzuki, T;Mitsuya, T
通讯作者：
Mitsuya, T
影响因子：
2.8
作者：
Rossignoli, Araceli E.;Fernandez, David;Blanco, Juan
通讯作者：
Blanco, Juan