Microglial activation in human diabetic retinopathy
Microglial activation in human diabetic retinopathy
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DOI：
10.1001/archophthalmol.2007.65
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发表时间：
2008-02-01
影响因子：
--
通讯作者：
Tso, Mark O. M.
中科院分区：
文献类型：
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作者：
Zeng, Hui-yang;Green, William Richard;Tso, Mark O. M.
目的：为探讨糖尿病视网膜病变中小胶质细胞的活化，采用免疫组化方法对13例糖尿病视网膜病变患者（21只眼）和9例正常人（10只眼）的石蜡切片进行了研究。在健康人眼中，小胶质细胞分散在视网膜内层。在糖尿病视网膜病变的眼睛，小胶质细胞的数量显着增加，并在不同阶段的疾病肥大。这些细胞聚集在视网膜血管系统周围，特别是扩张的静脉、微动脉瘤、视网膜内血管瘤、棉絮斑、视神经以及视网膜和玻璃体新生血管。在一些患有黄斑囊样水肿的视网膜中，小胶质细胞浸润视网膜外和视网膜下腔。结论：小胶质细胞在糖尿病视网膜病变和视神经病变的不同阶段被激活。小胶质细胞血管周围炎是糖尿病视网膜病变的一个显著特征。临床意义：小胶质细胞和小胶质细胞血管周围炎可能在糖尿病视网膜病变的血管病变和神经病变中发挥作用。
Objective: To investigate microglial activation in human diabetic retinopathy.Methods: Paraffin sections from 21 eyes of 13 patients with diabetic background, preproliferative, or proliferative retinopathies and 10 normal eyes of 9 individuals were studied with immunolabeling of microglia with antibodies against HLA-DR antigen, CD45, or CD68.Results: In the healthy human eyes, ramified microglial cells were scattered in the inner retinal layers. In eyes with diabetic retinopathy, the microglia were markedly increased in number and were hypertrophic at different stages of the disease. These cells clustered around the retinal vasculature, especially the dilated veins, microaneurysms, intraretinal hemorrhages, cotton-wool spots, optic nerve, and retinal and vitreal neovascularization. In some retinas with cystoid macular edema, microglia infiltrated the outer retina and subretinal space. Cells in the epiretinal membrane were also labeled with microglial markers.Conclusions: Microglia were activated at different stages of human diabetic retinopathy and optic neuropathy. Microglial perivasculitis was a prominent feature of the disease process.Clinical Relevance: Activated microglia and microglial perivasculitis may play a role in vasculopathy and neuropathy in diabetic retinopathy.