Physicochemical, cytotoxicity and in vivo biocompatibility of a high-plasticity calcium-silicate based material
Physicochemical, cytotoxicity and in vivo biocompatibility of a high-plasticity calcium-silicate based material
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DOI：
10.1038/s41598-019-40365-4
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发表时间：
2019-03-08
影响因子：
4.6
通讯作者：
Nogueira Leal Silva, Emmanuel Joao
中科院分区：
文献类型：
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作者：
Ferreira, Claudio M. A.;Sassone, Luciana M.;Nogueira Leal Silva, Emmanuel Joao
本研究旨在评价MTA Repair HP（MTA HP）和白色MTA（WMTA）的理化性质、细胞毒性和体内生物相容性。对固化时间、流动性、不透射线性和水溶性进行了评估。对于细胞毒性试验，将原代人成骨细胞暴露于两种材料洗脱液的几种稀释液中。MTT法、细胞凋亡法、细胞粘附法检测细胞凋亡率。通过使用不同染色技术的组织学分析来评价体内生物相容性。MTA HP与WMTA在凝固时间、不透射线性、溶解度和吸水率方面无显著差异（P>0.05）。然而，与WMTA相比，MTA HP显示出显著更高的流量（P
The purpose of this work was to evaluate the physicochemical properties, the cytotoxicity and in vivo biocompatibility of MTA Repair HP (MTA HP) and White MTA (WMTA). The setting time, flow, radiopacity and water solubility were assessed. To the cytotoxicity assay, primary human osteoblast cells were exposed to several dilutions of both materials eluates. MTT assay, apoptosis assay and cell adhesion assay were performed. The in vivo biocompatibility was evaluated through histological analysis using different staining techniques. No differences were observed between MTA HP and WMTA for setting time, radiopacity, solubility and water absorption (P>0.05). However, MTA HP showed a significantly higher flow when compared to WMTA (P