A NEW TECHNIQUE FOR RETARDING FADING OF FLUORESCENCE - DPX-BME
A NEW TECHNIQUE FOR RETARDING FADING OF FLUORESCENCE - DPX-BME
复制标题
DOI：
10.3109/10520298509113903
复制
发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
FILION, WG
中科院分区：
文献类型：
--
作者：
FRANKLIN, AL;FILION, WG
抗氧化剂β-巯基乙醇(BME)与永久性安装剂DPX(DPX-BME)一起使用，可以延缓米曲霉素、丫啶橙和Hoechst 33258染色的鸡红细胞的荧光褪色，每个都有不同程度的抑制。与单独使用DPX相比，DPX-BME对所有染料的初始荧光强度都更强。固定在DPX-BME中的样品具有强烈的荧光和良好的形态；如果在黑暗中保存，它们可以无限期储存而不会变质。用DPX-BME延缓荧光的褪色有利于用荧光细胞光度法定量DNA。
The antioxidant .beta.-mercaptoethanol (BME) used in conjunction with the permanent mountant DPX (DPX-BME) retarded fluorescent fading of mithramycin, acridine orange and Hoechst 33258 stained chicken erythrocytes, each to a varying degree. The initial fluorescence of all dyes examined was more intense with DPX-BME than with DPX alone. Specimens mounted in DPX-BME showed strong fluorescence and excellent morphology; if kept in the dark, they could be stored indefinitely without deterioration. Retarding fading of fluorescence with DPX-BME facilitated quantitation of DNA using fluorescence cytophotometry.